Genome location: Pf3D7_11_v3:1,718,938..1,725,224(-)
Genome classification: Core
Product Description: AP2 domain transcription factor AP2-O
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0003700 | DNA-binding transcription factor activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006355 | regulation of transcription, DNA-templated |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.48 | 0.85 |
| Trophozoite | not expressed | 0.12 | 0.93 |
| Schizont | expressed | 0.99 | 0.70 |
| Gametocyte | expressed | 1.45 | 0.39 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1143100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.406 | MFS: -2.557 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_0907300)
Modification: Disrupted | RMgm-4388
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHT5
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 31 | 83 | 62 | 97 |
| disruptive | 157 | 402 | 213 | 405 |
| missense | 82 | 293 | 175 | 306 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 49 | 179 | 74 | 110 |
| disruptive | 266 | 677 | 281 | 510 |
| missense | 154 | 510 | 213 | 331 |
PlasmoDB Total SNPs: 366
Non-coding: 101 | Synonymous: 128 | Nonsynonymous: 136 | Stop Codon: 1
Protein Length: 1604 | Molecular Weight (kDa): 186.13
UniProt ID(s): Q8IHT5
PDB ID(s): None
Isoelectric Point: 4.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001471 | AP2/ERF domain |
| PFam | PF00847 | AP2/ERF domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 34268135 | The Transcription Factor PfAP2-O Influences Virulence Gene Transcription andSexual Development in Plasmodium falciparum. | Cubillos EFG, Prata IO, ..., Wunderlich G | https://pubmed.ncbi.nlm.nih.gov/34268135/ |
| 35947628 | Identification of a novel AP2 transcription factor in zygotes with an essentialrole in Plasmodium ookinete development | Nishi T, Kaneko I, Iwanaga S, Yuda M | 10.1371/journal.ppat.1010510 |
| 20025671 | Transcription factor AP2-Sp and its target genes in malarial sporozoites | Yuda M, Iwanaga S, ..., Kaneko I | 10.1111/j.1365-2958.2009.07005.x |
| 19220746 | Identification of a transcription factor in the mosquito-invasive stage of malaria parasites | Yuda M, Iwanaga S, ..., Kaneko I | 10.1111/j.1365-2958.2009.06609.x |
| 26018192 | Genome-Wide Identification of the Target Genes of AP2-O, a Plasmodium AP2-FamilyTranscription Factor | Kaneko I, Iwanaga S, ..., Yuda M | 10.1371/journal.ppat.1004905 |
| 28081440 | A Knockout Screen of ApiAP2 Genes Reveals Networks of Interacting TranscriptionalRegulators Controlling the Plasmodium Life Cycle | Modrzynska K, Pfander C, ..., Billker O | 10.1016/j.chom.2016.12.003 |