Genome location: Pf3D7_11_v3:1,731,176..1,734,347(-)
Genome classification: Core
Product Description: DNA-directed RNA polymerases I and III subunit RPAC1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005736 | RNA polymerase I complex |
| Component | GO:0005666 | RNA polymerase III complex |
| Process | GO:0042797 | tRNA transcription by RNA polymerase III |
| Process | GO:0006360 | transcription by RNA polymerase I |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.83 | 0.61 |
| Trophozoite | expressed | 0.77 | 0.59 |
| Schizont | expressed | 0.23 | 0.85 |
| Gametocyte | expressed | 0.59 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1143300
Resistome Missense Mutations: Q95E
Resistome Compounds with Missense Mutations: SJ733
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.129 | MFS: -2.889 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHT3
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102180
Most Similar Human Ortholog: O15160
TM-align score: 0.95 | RMSD: 1.40
Seq Identity: 0.33 | Length: 328 / 333
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000171453 | RNA polymerase I and III subunit C |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 103
Non-coding: 83 | Synonymous: 12 | Nonsynonymous: 7 | Stop Codon: 1
Protein Length: 333 | Molecular Weight (kDa): 38.963
UniProt ID(s): Q8IHT3
PDB ID(s): None
Isoelectric Point: 7.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011262 | DNA-directed RNA polymerase, insert domain |
| InterPro | IPR011263 | DNA-directed RNA polymerase, RpoA/D/Rpb3-type |
| InterPro | IPR033901 | DNA-directed RNA polymerases I and III subunit AC40 |
| InterPro | IPR036603 | RNA polymerase, RBP11-like subunit |
| InterPro | IPR036643 | DNA-directed RNA polymerase, insert domain superfamily |
| PFam | PF01000 | DNA-directed RNA polymerase, insert domain |
| PFam | PF01193 | DNA-directed RNA polymerase, RpoA/D/Rpb3-type |
| Superfamily | SSF55257 | RNA polymerase, RBP11-like subunit |
| Superfamily | SSF56553 | DNA-directed RNA polymerase, insert domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 2203770 | ABF1 binding sites in yeast RNA polymerase genes | Della Seta F, Treich I, Buhler JM, Sentenac A | https://pubmed.ncbi.nlm.nih.gov/2203770/ |
| 7929437 | High conservation of subunit composition of RNA polymerase I(A) between yeast andmouse and the molecular cloning of mouse RNA polymerase I 40-kDa subunit RPA40 | Song CZ, Hanada K, ..., Muramatsu M | https://pubmed.ncbi.nlm.nih.gov/7929437/ |
| 2830265 | RPA190, the gene coding for the largest subunit of yeast RNA polymerase A | Memet S, Gouy M, ..., Buhler JM | https://pubmed.ncbi.nlm.nih.gov/2830265/ |
| 10079952 | [Molecular cloning and characteristics of rpc19+ and rpc40+ Schizosaccharomycespombe genes, coding for common subunits of nuclear RNA polymerase I and III] | Shpakovskii GV, Shematorova EK | https://pubmed.ncbi.nlm.nih.gov/10079952/ |
| 16730080 | Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei | Nguyen TN, Schimanski B, ..., Gunzl A | 10.1016/j.molbiopara.2006.02.023 |
| 34055890 | Specific Features of RNA Polymerases I and III: Structure and Assembly | Turowski TW, Boguta M | 10.3389/fmolb.2021.680090 |
| 3815519 | RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C | Mann C, Buhler JM, Treich I, Sentenac A | 10.1016/0092-8674(87)90241-8 |
| 8164651 | Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing | Hermann-Le Denmat S, Werner M, Sentenac A, Thuriaux P | 10.1128/mcb.14.5.2905-2913.1994 |
| 2112240 | A conjugation-specific gene (cnjC) from Tetrahymena encodes a protein homologous to yeast RNA polymerase subunits (RPB3, RPC40) and similar to a portion of theprokaryotic RNA polymerase alpha subunit (rpoA) | Martindale DW | 10.1093/nar/18.10.2953 |
| 10541858 | Rpc19 and Rpc40, two alpha-like subunits shared by nuclear RNA polymerases I and III, are interchangeable between the fission and budding yeasts | Shpakovski GV, Shematorova EK | 10.1007/s002940050492 |
| 25931562 | Retrotransposons. An RNA polymerase III subunit determines sites of retrotransposon integration | Bridier-Nahmias A, Tchalikian-Cosson A, ..., Lesage P | 10.1126/science.1259114 |
| 10628857 | Isolation and characterization of the fission yeast gene rpa42+, which encodes asubunit shared by RNA polymerases I and III | Imazawa Y, Imai K, ..., Nogi Y | 10.1007/pl00013818 |
| 8516295 | Interactions between three common subunits of yeast RNA polymerases I and III | Lalo D, Carles C, Sentenac A, Thuriaux P | 10.1073/pnas.90.12.5524 |
| 9540830 | Cloning and characterization of the human RNA polymerase I subunit hRPA40 | Dammann R, Pfeifer GP | 10.1016/s0167-4781(97)00206-6 |