PF3D7_1143900

Genome location: Pf3D7_11_v3:1,751,384..1,753,416(-)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent (S)-NAD(P)H-hydrate dehydratase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.1.50 (Hydroxyethylthiazole kinase);4.2.1.93 (ATP-dependent NAD(P)H-hydrate dehydratase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0047453	ATP-dependent NAD(P)H-hydrate dehydratase activity
Process	GO:0110051	metabolite repair

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.08	0.96
Trophozoite	expressed	0.10	0.94
Schizont	expressed	0.02	0.99
Gametocyte	expressed	0.27	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1143900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.572 | MFS: -3.067 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0905100)

Relative Growth Rate: 0.97 ± 0.14
Confidence: 5.34

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0905100)

Modification: Disrupted | RMgm-2496

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IHS6

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.11) with 3RRE (Global RMSD=3.05)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.1.50	hydroxyethylthiazole kinase	PCMB2-Aminohydroxyethylthiazole
4.2.1.93	ATP-dependent NAD(P)H-hydrate dehydratase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100751

Most Similar Human Ortholog: A0A7P0T9D8

TM-align score: 0.87 | RMSD: 2.12

Seq Identity: 0.27 | Length: 306 / 391

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000213995	NAD(P)HX dehydratase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	21	18	21
disruptive	7	44	25	63
missense	7	43	25	56

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	43	23	23
disruptive	10	94	47	103
missense	9	82	41	71

PlasmoDB Total SNPs: 184

Non-coding: 160 | Synonymous: 13 | Nonsynonymous: 11 | Stop Codon: 0

Protein Information

Protein Length: 391 | Molecular Weight (kDa): 45.068

UniProt ID(s): Q8IHS6

PDB ID(s): None

Isoelectric Point: 8.76

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000631	ATP-dependent (S)-NAD(P)H-hydrate dehydratase
InterPro	IPR029056	Ribokinase-like
PFam	PF01256	ATP-dependent (S)-NAD(P)H-hydrate dehydratase
Superfamily	SSF53613	Ribokinase-like