Genome location: Pf3D7_11_v3:1,770,414..1,772,184(+)
Genome classification: Core
Product Description: transcription initiation factor IIF subunit beta, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB): None
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.42 | 0.79 |
| Trophozoite | expressed | 0.55 | 0.67 |
| Schizont | expressed | 0.14 | 0.90 |
| Gametocyte | expressed | 0.39 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1144600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.21 | MFS: -2.701 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0904400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0904400)
Modification: Disrupted | RMgm-2492
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHS1
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 21 | 15 | 19 |
| disruptive | 6 | 38 | 16 | 38 |
| missense | 3 | 38 | 15 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 38 | 21 | 25 |
| disruptive | 12 | 71 | 32 | 66 |
| missense | 7 | 63 | 31 | 47 |
PlasmoDB Total SNPs: 55
Non-coding: 37 | Synonymous: 13 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 317 | Molecular Weight (kDa): 36.668
UniProt ID(s): Q8IHS1
PDB ID(s): None
Isoelectric Point: 10.01
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 15572698 | Amino acid substitutions in yeast TFIIF confer upstream shifts in transcriptioninitiation and altered interaction with RNA polymerase II | Ghazy MA, Brodie SA, ..., Ponticelli AS | 10.1128/MCB.24.24.10975-10985.2004 |
| 23535894 | Crystal structure of human Karyopherin beta2 bound to the PY-NLS of Saccharomycescerevisiae Nab2 | Soniat M, Sampathkumar P, ..., Almo SC | 10.1007/s10969-013-9150-1 |
| 14522989 | Yeast RNA polymerase II lacking the Rpb9 subunit is impaired for interaction withtranscription factor IIF | Ziegler LM, Khaperskyy DA, Ammerman ML, Ponticelli AS | 10.1074/jbc.M309656200 |
| 15616156 | Tfg3, a subunit of the general transcription factor TFIIF in Schizosaccharomycespombe, functions under stress conditions | Kimura M, Ishihama A | 10.1093/nar/gkh1000 |
| 18362165 | Functions of Saccharomyces cerevisiae TFIIF during transcription start siteutilization | Khaperskyy DA, Ammerman ML, Majovski RC, Ponticelli AS | 10.1128/MCB.02272-07 |
| 22095626 | Structural and binding studies of the C-terminal domains of yeast TFIIF subunitsTfg1 and Tfg2 | Kilpatrick AM, Koharudin LM, Calero GA, Gronenborn AM | 10.1002/prot.23217 |