Genome location: Pf3D7_11_v3:1,772,009..1,774,198(-)
Genome classification: Core
Product Description: apicoplast import protein Tic20, putative
SignalP Peptide: MCRFLTYHYILIFCIIIKNVFS
# Transmembrane Domains: 5
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Process | GO:0017038 | protein import |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.32 | 0.80 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | expressed | 0.24 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1144700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.003 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0904300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0904300)
Modification: Disrupted | RMgm-2491
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHS0
"Best" AlphaFill ligand hit: LFA (lipid fragment, Local RMSD=0.09) with 7AKW (Global RMSD=10.05)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 24 | 10 | 15 |
| disruptive | 2 | 16 | 13 | 27 |
| missense | 2 | 16 | 13 | 26 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 41 | 11 | 20 |
| disruptive | 9 | 38 | 20 | 55 |
| missense | 7 | 37 | 18 | 47 |
PlasmoDB Total SNPs: 167
Non-coding: 143 | Synonymous: 19 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 259 | Molecular Weight (kDa): 30.78
UniProt ID(s): Q8IHS0
PDB ID(s): None
Isoelectric Point: 9.92
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005691 | Chloroplast protein import component Tic20 |
| PFam | PF16166 | Chloroplast protein import component Tic20 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18757752 | Toxoplasma gondii Tic20 is essential for apicoplast protein import. | van Dooren GG, Tomova C, ..., Striepen B | 10.1073/pnas.0803862105 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 28595540 | Apicoplast Import Protein Tic20 A Promising Therapeutic Molecular Target forPlasmodium falciparum: An In Silico Approach for Therapeutic Intervention | C P K Srimath-Tirumala-Peddinti R, Kusuma SS, Nammi D, Neelapu NRR | 10.2174/1871526517666170606113133 |
| 30206320 | High-density SNP-based QTL mapping and candidate gene screening for yield-relatedblade length and width in Saccharina japonica (Laminariales, Phaeophyta) | Wang X, Chen Z, ..., Duan D | 10.1038/s41598-018-32015-y |
| 31426420 | Verification of the Saccharina japonica Translocon Tic20 and its Localization inthe Chloroplast Membrane in Diatoms | Chen Z, Wang X, ..., Duan D | 10.3390/ijms20164000 |