Genome location: Pf3D7_11_v3:1,777,078..1,779,609(-)
Genome classification: Core
Product Description: ras-related protein Rab-6
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.5.2 (Small monomeric GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:0005829 | cytosol |
| Component | GO:0012505 | endomembrane system |
| Component | GO:0005802 | trans-Golgi network |
| Function | GO:0003924 | GTPase activity |
| Process | GO:0006891 | intra-Golgi vesicle-mediated transport |
| Process | GO:0006886 | intracellular protein transport |
| Process | GO:0042147 | retrograde transport, endosome to Golgi |
| Process | GO:0006890 | retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.15 | 0.92 |
| Trophozoite | expressed | 0.35 | 0.78 |
| Schizont | expressed | 0.52 | 0.68 |
| Gametocyte | possibly expressed | 0.21 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1144900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.78 | MFS: -2.414 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0904100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHR8
"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.07) with 3LAW (Global RMSD=2.14)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.2 | small monomeric GTPase | GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100976
Most Similar Human Ortholog: C9JU14
TM-align score: 0.96 | RMSD: 0.81
Seq Identity: 0.72 | Length: 83 / 207
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000154917 | RAB6B, member RAS oncogene family |
| ENSG00000175582 | RAB6A, member RAS oncogene family |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 1 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 1 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 147
Non-coding: 139 | Synonymous: 7 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 207 | Molecular Weight (kDa): 23.61
UniProt ID(s): Q26000, Q7KPX8, Q8IHR8, Q94663
PDB ID(s): None
Isoelectric Point: 6.48
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001806 | Small GTPase |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00071 | Small GTPase |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12657463 | The Plasmodium falciparum family of Rab GTPases. | Quevillon E, Spielmann T, ..., Langsley G | 10.1016/s0378-1119(03)00381-0 |
| 17428488 | Re-assessing the locations of components of the classical vesicle-mediatedtrafficking machinery in transfected Plasmodium falciparum. | Adisa A, Frankland S, ..., Tilley L | 10.1016/j.ijpara.2007.02.009 |
| 18284574 | Spatial dissection of the cis- and trans-Golgi compartments in the malariaparasite Plasmodium falciparum. | Struck NS, Herrmann S, ..., Gilberger TW | 10.1111/j.1365-2958.2008.06125.x |
| 36523508 | An imaging-based RNA interference screen for modulators of the Rab6-mediatedGolgi-to-ER pathway in mammalian cells | Heffernan LF, Suckrau PM, ..., Simpson JC | 10.3389/fcell.2022.1050190 |
| 37844085 | A Rab6 to Rab11 transition is required for dense-core granule and exosome biogenesis in Drosophila secondary cells | Wells A, Mendes CC, ..., Wilson C | 10.1371/journal.pgen.1010979 |
| 36847222 | MicroRNA miR-252-5p regulates the Notch signaling pathway by targeting Rab6 in Drosophila wing development | Lim DH, Choi MS, Jeon JW, Lee YS | 10.1111/1744-7917.13188 |