Genome location: Pf3D7_11_v3:1,820,807..1,823,494(-)
Genome classification: Core
Product Description: ribosome assembly protein 4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005730 | nucleolus |
| Component | GO:0005634 | nucleus |
| Process | GO:0000027 | ribosomal large subunit assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.38 | 0.81 |
| Trophozoite | expressed | 0.35 | 0.80 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | expressed | 0.24 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1146000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.13 | MFS: -2.961 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHQ8
"Best" AlphaFill ligand hit: SIW (keggin (sta), Local RMSD=0.03) with 6Y7O (Global RMSD=3.52)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101916
Most Similar Human Ortholog: Q9NVX2
TM-align score: 0.80 | RMSD: 2.24
Seq Identity: 0.42 | Length: 409 / 645
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000073536 | notchless homolog 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 45 | 19 | 42 |
| disruptive | 21 | 126 | 55 | 136 |
| missense | 15 | 114 | 51 | 120 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 85 | 24 | 50 |
| disruptive | 45 | 246 | 80 | 186 |
| missense | 33 | 219 | 66 | 117 |
PlasmoDB Total SNPs: 142
Non-coding: 74 | Synonymous: 39 | Nonsynonymous: 29 | Stop Codon: 0
Protein Length: 645 | Molecular Weight (kDa): 73.128
UniProt ID(s): Q8IHQ8
PDB ID(s): None
Isoelectric Point: 7.59
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001680 | WD40 repeat |
| InterPro | IPR012972 | NLE |
| InterPro | IPR019775 | WD40 repeat, conserved site |
| InterPro | IPR020472 | G-protein beta WD-40 repeat |
| InterPro | IPR036322 | WD40-repeat-containing domain superfamily |
| PFam | PF00400 | WD40 repeat |
| PFam | PF08154 | NLE |
| Superfamily | SSF50978 | WD40-repeat-containing domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 25827419 | Essential gene discovery in the basidiomycete Cryptococcus neoformans for antifungal drug target prioritization | Ianiri G, Idnurm A | 10.1128/mBio.02334-14 |
| 33266193 | Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly | Paternoga H, Fruh A, ..., Hurt E | 10.3390/ijms21239108 |
| 19737519 | Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits | Ulbrich C, Diepholz M, ..., Hurt E | 10.1016/j.cell.2009.06.045 |
| 26601951 | The Crystal Structure of the Ubiquitin-like Domain of Ribosome Assembly Factor Ytm1 and Characterization of Its Interaction with the AAA-ATPase Midasin | Romes EM, Sobhany M, Stanley RE | 10.1074/jbc.M115.693259 |
| 30460895 | The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factorRea1 | Sosnowski P, Urnavicius L, ..., Schmidt H | 10.7554/eLife.39163 |
| 25404745 | A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation | Bassler J, Paternoga H, ..., Hurt E | 10.1083/jcb.201408111 |
| 23874617 | Studies on the assembly characteristics of large subunit ribosomal proteins in S. cerevisae | Ohmayer U, Gamalinda M, ..., Milkereit P | 10.1371/journal.pone.0068412 |