PF3D7_1146600 (ORP1)

Genome location: Pf3D7_11_v3:1,832,152..1,839,819(+)

Genome classification: Core

Function and Localization

Product Description: oocyst rupture protein 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0071944	cell periphery
Component	GO:0005634	nucleus
Function	GO:0003677	DNA binding
Process	GO:0006355	regulation of transcription, DNA-templated

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.39	0.85
Trophozoite	expressed	0.56	0.68
Schizont	expressed	1.52	0.38
Gametocyte	expressed	0.38	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1146600

Resistome Missense Mutations: E277Q, E280Q

Resistome Compounds with Missense Mutations: MMV024114, Proguanil

Resistome # Samples with Disruptive Mutations: 7 (2 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.157 | #Insertions: 6

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0902500)

Relative Growth Rate: 0.89 ± 0.08
Confidence: 6.38

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0902500)

Modification: Mutated | RMgm-4061

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IHQ2

"Best" AlphaFill ligand hit: SVR (suramin, Local RMSD=0.74) with 7AH8 (Global RMSD=1.44)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101084

Most Similar Human Ortholog: F8VSL3

TM-align score: 0.51 | RMSD: 3.40

Seq Identity: 0.43 | Length: 128 / 1301

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000120837	nuclear transcription factor Y subunit beta

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	50	20	62
disruptive	71	162	90	216
missense	43	129	79	187

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	21	76	57	84
disruptive	125	379	184	401
missense	74	301	149	255

PlasmoDB Total SNPs: 488

Non-coding: 297 | Synonymous: 93 | Nonsynonymous: 96 | Stop Codon: 2

Protein Information

Protein Length: 1301 | Molecular Weight (kDa): 150.983

UniProt ID(s): Q8IHQ2, Q8WSK5

PDB ID(s): None

Isoelectric Point: 4.35

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain
InterPro	IPR009072	Histone-fold
PFam	PF00808	Transcription factor CBF/NF-Y/archaeal histone domain
Superfamily	SSF47113	Histone-fold

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
22804732	The PfNF-YB transcription factor is a downstream target of melatonin and cAMPsignalling in the human malaria parasite Plasmodium falciparum.	Lima WR, Moraes M, ..., Garcia CR	10.1111/j.1600-079X.2012.01021.x
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
27982038	Release of Plasmodium sporozoites requires proteins with histone-folddimerization domains.	Curra C, Gessmann R, ..., Siden-Kiamos I	10.1038/ncomms13846
36126573	Structural elucidation of a novel polysaccharide from Ophiopogonis Radix and itsself-assembly mechanism in aqueous solution	Wang B, Huang B, ..., Feng L	10.1016/j.foodchem.2022.134165
36461309	Polysaccharide ORP-1 isolated from Oudemansiella raphanipes amelioratesage-associated intestinal epithelial barrier dysfunction in Caco-2 cellsmonolayer	Pan WJ, Shi LL, ..., Chen Y	10.1016/j.foodres.2022.112038