Genome location: Pf3D7_11_v3:1,858,329..1,867,357(-)
Genome classification: Core
Product Description: sporozoite and liver stage asparagine-rich protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Process | GO:0010468 | regulation of gene expression |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.98 |
| Trophozoite | not expressed | 0.09 | 0.95 |
| Schizont | possibly expressed | 0.30 | 0.81 |
| Gametocyte | not expressed | 0.24 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1147000
Resistome Missense Mutations: H2634Q, Y2691N
Resistome Compounds with Missense Mutations: MMV1454442, MMV982556
Resistome # Samples with Disruptive Mutations: 2 (2 missense, 1 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.719 | #Insertions: 10
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_0903500)
Modification: Disrupted | RMgm-4068
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 29 | 149 | 77 | 163 |
| disruptive | 142 | 503 | 250 | 534 |
| missense | 108 | 422 | 216 | 439 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 56 | 265 | 124 | 206 |
| disruptive | 291 | 990 | 379 | 826 |
| missense | 196 | 801 | 298 | 522 |
PlasmoDB Total SNPs: 402
Non-coding: 27 | Synonymous: 188 | Nonsynonymous: 187 | Stop Codon: 0
Protein Length: 2940 | Molecular Weight (kDa): 348.041
UniProt ID(s): Q8IHP9
PDB ID(s): None
Isoelectric Point: 6.83
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18551171 | A sporozoite asparagine-rich protein controls initiation of Plasmodium liverstage development. | Silvie O, Goetz K, Matuschewski K | 10.1371/journal.ppat.1000086 |
| 25407681 | A genetically attenuated malaria vaccine candidate based on P. falciparumb9/slarp gene-deficient sporozoites | van Schaijk BC, Ploemen IH, ..., Sauerwein RW | 10.7554/eLife.03582 |
| 32434847 | A double-blind, placebo-controlled phase 1/2a trial of the genetically attenuatedmalaria vaccine PfSPZ-GA1 | Roestenberg M, Walk J, ..., Sauerwein RW | 10.1126/scitranslmed.aaz5629 |
| 36622145 | Targetome Analysis of Malaria Sporozoite Transcription Factor AP2-Sp Reveals ItsRole as a Master Regulator | Yuda M, Kaneko I, ..., Nishi T | 10.1128/mbio.02516-22 |
| 24657782 | The Plasmodium protein P113 supports efficient sporozoite to liver stageconversion in vivo | Offeddu V, Rauch M, Silvie O, Matuschewski K | 10.1016/j.molbiopara.2014.03.002 |