PF3D7_1147200

Genome location: Pf3D7_11_v3:1,872,795..1,881,752(+)

Genome classification: Core

Function and Localization

Product Description: tubulin--tyrosine ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.3.2.25 (Tubulin--tyrosine ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005929	cilium
Function	GO:0015631	tubulin binding
Function	GO:0070740	tubulin-glutamic acid ligase activity
Process	GO:0000226	microtubule cytoskeleton organization
Process	GO:0018095	protein polyglutamylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	not expressed	0.02	0.99
Schizont	not expressed	0.63	0.67
Gametocyte	expressed	1.54	0.43

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1147200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.94 | #Insertions: 6

PlasmoGEM Phenotype: Insufficient data (Pb ortholog: PBANKA_0901900)

Relative Growth Rate: 0.51 ± 0.75
Confidence: 1.97

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.3.2.25	tubulin-tyrosine ligase	ADPAMPNEML-PheL-TyrL-DopatyraminestathminphosphateL-Tyr-GlyL-Tyr-L-GluL-Ala-L-Tyr...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_108606

Most Similar Human Ortholog: Not available

TM-align score: 0.00 | RMSD: 0.00

Seq Identity: 0.00 | Length: / 2755

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000175764	tubulin tyrosine ligase like 11

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	26	130	86	169
disruptive	116	561	257	546
missense	73	493	223	464

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	48	278	95	200
disruptive	241	1057	379	761
missense	148	879	307	490

PlasmoDB Total SNPs: 428

Non-coding: 86 | Synonymous: 164 | Nonsynonymous: 178 | Stop Codon: 0

Protein Information

Protein Length: 2755 | Molecular Weight (kDa): 325.326

UniProt ID(s): Q8IHP8

PDB ID(s): None

Isoelectric Point: 9.63

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	SSF56059	N/A