Genome location: Pf3D7_11_v3:1,889,494..1,892,695(-)
Genome classification: Core
Product Description: protein farnesyltransferase subunit beta
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.5.1.58 (Protein farnesyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005965 | protein farnesyltransferase complex |
| Function | GO:0004660 | protein farnesyltransferase activity |
| Function | GO:0008270 | zinc ion binding |
| Process | GO:0018343 | protein farnesylation |
| Process | GO:0018342 | protein prenylation |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.08 | 0.95 |
| Schizont | expressed | 0.01 | 0.99 |
| Gametocyte | possibly expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1147500
Resistome Missense Mutations: A515V, A515T, Y837N
Resistome Compounds with Missense Mutations: MMV019066
Resistome # Samples with Disruptive Mutations: 6 (6 missense, 6 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.128 | MFS: -3.062 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0901600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHP6
"Best" AlphaFill ligand hit: PPV (pyrophosphate, Local RMSD=0.12) with 7RN5 (Global RMSD=3.29)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.5.1.58 | protein farnesyltransferase | ED5EDTACVFMCVIMDPI-1FTI-276R115777FTI-277KTSCVAMKTSCVFMKTSCVIAKTSCVIF... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P22007 | Protein farnesyltransferase subunit beta/geranylgeranyltransferase type-1 subunit alpha | Saccharomyces cerevisiae | OrthoDB, OrthoMCL | 3-(4-Chlorophenyl)-4-cyano-5-(isopropylthio)thiophene-2-carboxylic acid (1a)3-(3-Chlorophenyl)-4-cyano-5-(isopropylthio)thiophene-2-carboxylic acid (1b)3-(2-Chlorophenyl)-4-cyano-5-(isopropylthio)thiophene-2-carboxylic acid (1c)3-(4-Fluorophenyl)-4-cyano-5-(isopropylthio)thiophene-2-carboxylic acid (1d)... |
| P53610 | Geranylgeranyl transferase type-1 subunit beta | OrthoDB | CHEMBL1075839 | |
| Q5EAD5 | Geranylgeranyl transferase type-1 subunit beta/Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha | Bos taurus | OrthoDB | A313326 Analogue 7A313326 Analogue 8A313326 Analogue 10A313326 Analogue 11... |
| P49356 | Protein farnesyltransferase subunit beta | Homo sapiens | OrthoDB | CHEMBL334749CHEMBL334749CHEMBL311310CHEMBL440520... |
| P53609 | Geranylgeranyl transferase type-1 subunit beta | OrthoDB | MSD-1CHEMBL99205CHEMBL327278CHEMBL102107... |
Ortholog Group (OrthoMCL): OG6_101706
Most Similar Human Ortholog: P49356
TM-align score: 0.78 | RMSD: 2.85
Seq Identity: 0.35 | Length: 367 / 923
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000125954 | CHURC1-FNTB readthrough |
| ENSG00000257365 | farnesyltransferase, CAAX box, beta |
Protein Length: 923 | Molecular Weight (kDa): 109.826
UniProt ID(s): A0FDU2, Q5EI71, Q5EI72, Q5EI74, Q8IHP6
PDB ID(s): None
Isoelectric Point: 7.21
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001330 | PFTB repeat |
| InterPro | IPR008930 | Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid |
| InterPro | IPR026872 | Protein farnesyltransferase subunit beta |
| PFam | PF00432 | PFTB repeat |
| Superfamily | SSF48239 | Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 61 | 31 | 45 |
| disruptive | 34 | 136 | 63 | 128 |
| missense | 34 | 135 | 61 | 120 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 31 | 97 | 42 | 48 |
| disruptive | 59 | 236 | 98 | 204 |
| missense | 55 | 216 | 94 | 163 |
PlasmoDB Total SNPs: 85
Non-coding: 15 | Synonymous: 28 | Nonsynonymous: 42 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12194969 | Protein farnesyltransferase and protein prenylation in Plasmodium falciparum. | Chakrabarti D, Da Silva T, ..., Allen CM | 10.1074/jbc.M202860200 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15661734 | Resistance to a protein farnesyltransferase inhibitor in Plasmodium falciparum. | Eastman RT, White J, ..., Van Voorhis WC | 10.1074/jbc.M413556200 |
| 29326268 | Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics. | Cowell AN, Istvan ES, ..., Winzeler EA | 10.1126/science.aan4472 |