Genome location: Pf3D7_11_v3:1,899,181..1,905,942(+)
Genome classification: Core
Product Description: membrane associated erythrocyte binding-like protein
SignalP Peptide: MGVLKHFFFLLFLYVNNTSA
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020007 | apical complex |
| Component | GO:0020008 | rhoptry |
| Function | GO:0046812 | host cell surface binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | nd | nd |
| Trophozoite | possibly expressed | nd | nd |
| Schizont | expressed | nd | nd |
| Gametocyte | possibly expressed | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1147800.2
Resistome Missense Mutations: A1697V
Resistome Compounds with Missense Mutations: AN13762
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.858 | #Insertions: 10
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0901300)
Modification: Tagged | RMgm-5216
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A3C7
"Best" AlphaFill ligand hit: LVV (4-[(4-methylphenyl)methyl]-1,4-thiazinane 1,1-dioxide, Local RMSD=6.05) with 5QSP (Global RMSD=14.19)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 68 | 38 | 84 |
| disruptive | 37 | 165 | 86 | 217 |
| missense | 33 | 152 | 76 | 193 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 124 | 67 | 87 |
| disruptive | 69 | 355 | 177 | 326 |
| missense | 52 | 288 | 158 | 235 |
PlasmoDB Total SNPs: 433
Non-coding: 46 | Synonymous: 300 | Nonsynonymous: 87 | Stop Codon: 0
Protein Length: 2055 | Molecular Weight (kDa): 243.213
UniProt ID(s): J7GRT8, Q078Q1, Q078Q2, Q078Q7, Q078Q8, Q078R2, Q078R3, Q078S0, Q7K5Q5, Q7K5Q6, Q8IHP3, Q8T5C7, Q8T7Y1
PDB ID(s): None
Isoelectric Point: 9.3
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR021620 | Erythrocyte binding antigen 175, C-terminal |
| PFam | PF11556 | Erythrocyte binding antigen 175, C-terminal |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15465047 | Conservation and developmental control of alternative splicing in maebl amongmalaria parasites. | Singh N, Preiser P, ..., Adams JH | 10.1016/j.jmb.2004.08.047 |
| 17688178 | Comparative analysis of molecular variation in Plasmodium falciparum and P.reichenowi maebl gene. | Verra F, Polley SD, Thomas AW, Conway DJ | https://pubmed.ncbi.nlm.nih.gov/17688178/ |
| 18509478 | The transmembrane isoform of Plasmodium falciparum MAEBL is essential for theinvasion of Anopheles salivary glands. | Saenz FE, Balu B, ..., Adams JH | 10.1371/journal.pone.0002287 |
| 28371168 | A MA1 and M AEBL are important for Plasmodium falciparum sporozoite infection ofthe liver | Yang ASP, Lopaticki S, ..., Boddey JA | 10.1111/cmi.12745 |
| 26169268 | Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoeliiInfection | Leite JA, Bargieri DY, ..., Costa FT | 10.1128/IAI.00262-15 |
| 26712012 | Dissecting the interface between apicomplexan parasite and host cell: Insightsfrom a divergent AMA-RON2 pair | Parker ML, Penarete-Vargas DM, ..., Boulanger MJ | 10.1073/pnas.1515898113 |
| 18950624 | Identification of the transcription initiation site reveals a novel transcriptstructure for Plasmodium falciparum maebl | Balu B, Blair PL, Adams JH | 10.1016/j.exppara.2008.10.003 |
| 35628522 | MAEBL Contributes to Plasmodium Sporozoite Adhesiveness | Sa M, Costa DM, ..., Tavares J | 10.3390/ijms23105711 |
| 17041140 | Developmental biology of sporozoite-host interactions in Plasmodium falciparummalaria: implications for vaccine design | Garcia JE, Puentes A, Patarroyo ME | 10.1128/CMR.00063-05 |
| 27130708 | Breadth of humoral response and antigenic targets of sporozoite-inhibitoryantibodies associated with sterile protection induced by controlled human malariainfection | Peng K, Goh YS, ..., Renia L | 10.1111/cmi.12608 |
| 33044985 | Whole genome sequencing of Plasmodium vivax isolates reveals frequent sequenceand structural polymorphisms in erythrocyte binding genes | Ford A, Kepple D, ..., Lo E | 10.1371/journal.pntd.0008234 |
| 19016774 | Role for the Plasmodium sporozoite-specific transmembrane protein S6 in parasitemotility and efficient malaria transmission | Steinbuechel M, Matuschewski K | 10.1111/j.1462-5822.2008.01252.x |
| 16154214 | Structural comparison of apical membrane antigen 1 orthologues and paralogues inapicomplexan parasites | Chesne-Seck ML, Pizarro JC, ..., Bentley GA | 10.1016/j.molbiopara.2005.07.007 |
| 15811533 | Targeted disruption of maebl in Plasmodium falciparum | Fu J, Saenz FE, ..., Adams JH | 10.1016/j.molbiopara.2004.12.017 |
| 17040128 | Two Plasmodium rhomboid proteases preferentially cleave different adhesinsimplicated in all invasive stages of malaria | Baker RP, Wijetilaka R, Urban S | 10.1371/journal.ppat.0020113 |
| 23224610 | Identification and expression of maebl, an erythrocyte-binding gene, inPlasmodium gallinaceum | Martinez C, Marzec T, ..., Sehgal RN | 10.1007/s00436-012-3211-4 |
| 33335684 | The protozoan parasite Toxoplasma gondii encodes a gamut of phosphodiesterasesduring its lytic cycle in human cells | Vo KC, Gunay-Esiyok O, Liem N, Gupta N | 10.1016/j.csbj.2020.11.024 |
| 38277340 | Longitudinal analysis of antibody responses to Plasmodium vivax sporozoiteantigens following natural infection | Thawornpan P, Nicholas J, ..., Chootong P | 10.1371/journal.pntd.0011907 |
| 29316918 | In silico epitope mapping and experimental evaluation of the Merozoite AdhesiveErythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate | Cravo P, Machado RB, ..., Costa FTM | 10.1186/s12936-017-2144-x |
| 37703302 | Preliminary characterization of Plasmodium vivax sporozoite antigens aspre-erythrocytic vaccine candidates | Nicholas J, De SL, ..., Adams JH | 10.1371/journal.pntd.0011598 |