PF3D7_1148000

Genome location: Pf3D7_11_v3:1,908,678..1,914,041(-)

Genome classification: Core

Function and Localization

Product Description: serine/threonine protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0005524	ATP binding
Function	GO:0004672	protein kinase activity
Function	GO:0004674	protein serine/threonine kinase activity
Process	GO:0007059	chromosome segregation
Process	GO:0035556	intracellular signal transduction
Process	GO:0018105	peptidyl-serine phosphorylation
Process	GO:0006468	protein phosphorylation
Process	GO:0001672	regulation of chromatin assembly or disassembly

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.10	0.95
Trophozoite	expressed	0.85	0.56
Schizont	expressed	0.24	0.85
Gametocyte	expressed	0.63	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1148000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -2.973 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0901100)

Relative Growth Rate: 0.04 ± 0.07
Confidence: 6.82

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IHP1

"Best" AlphaFill ligand hit: HV2 (1,1-bis(oxidanylidene)thietan-3-ol, Local RMSD=0.03) with 6QAL (Global RMSD=7.06)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9UKI8	Serine/threonine-protein kinase tousled-like 1	Homo sapiens	OrthoMCL BLAST	CHEMBL3775181Staurosporine, 8Staurosporine, 8Staurosporine, 8...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101432

Most Similar Human Ortholog: J3QLK5

TM-align score: 0.49 | RMSD: 4.41

Seq Identity: 0.27 | Length: 335 / 1429

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000146872	tousled like kinase 2
ENSG00000198586	tousled like kinase 1

Protein Information

Protein Length: 1429 | Molecular Weight (kDa): 170.705

UniProt ID(s): Q8IHP1

PDB ID(s): None

Isoelectric Point: 9.32

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR017441	Protein kinase, ATP binding site
PFam	PF00069	Protein kinase domain
Superfamily	SSF56112	Protein kinase-like domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	12	75	36	62
disruptive	53	148	60	146
missense	38	123	53	120

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	23	132	48	62
disruptive	100	231	89	175
missense	65	180	79	134

PlasmoDB Total SNPs: 134

Non-coding: 53 | Synonymous: 35 | Nonsynonymous: 45 | Stop Codon: 1