Genome location: Pf3D7_11_v3:1,948,696..1,969,412(+)
Genome classification: Core
Product Description: antigen 332, DBL-like protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0033644 | host cell membrane |
| Component | GO:0005634 | nucleus |
| Function | GO:0046789 | host cell surface receptor binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0098609 | cell-cell adhesion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.82 | 0.72 |
| Trophozoite | expressed | 2.99 | 0.12 |
| Schizont | expressed | 0.53 | 0.76 |
| Gametocyte | not expressed | 0.25 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1149000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.293 | #Insertions: 8
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 27 | 133 | 62 | 148 |
| disruptive | 182 | 709 | 385 | 850 |
| missense | 137 | 584 | 300 | 691 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 46 | 211 | 80 | 202 |
| disruptive | 299 | 1286 | 498 | 1100 |
| missense | 223 | 1037 | 391 | 808 |
PlasmoDB Total SNPs: 1574
Non-coding: 151 | Synonymous: 648 | Nonsynonymous: 774 | Stop Codon: 1
Protein Length: 6093 | Molecular Weight (kDa): 689.283
UniProt ID(s): A0A0K2RVQ3, A0A0K2RVU1, A0A0K2RVV7, A0A0K2RWC5, I6M224, Q25751, Q58HL9, Q58HM0, Q7JPX5, Q7JPX6, Q8IHN4, Q9U459
PDB ID(s): None
Isoelectric Point: 3.56
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006763 | Antigen 332 repeat |
| InterPro | IPR008602 | Duffy-antigen binding |
| PFam | PF04671 | Antigen 332 repeat |
| PFam | PF05424 | Duffy-antigen binding |
| Superfamily | SSF140924 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17534427 | A novel DBL-domain of the P. falciparum 332 molecule possibly involved inerythrocyte adhesion. | Moll K, Chene A, ..., Chen Q | 10.1371/journal.pone.0000477 |
| 18832660 | Functional alteration of red blood cells by a megadalton protein of Plasmodiumfalciparum. | Glenister FK, Fernandez KM, ..., Cooke BM | 10.1182/blood-2008-05-157735 |
| 23185236 | Plasmodium falciparum antigen 332 is a resident peripheral membrane protein ofMaurer's clefts. | Nilsson S, Angeletti D, ..., Moll K | 10.1371/journal.pone.0046980 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 32184257 | Role of Plasmodium falciparum Protein G EXP07 in Maurer's Cleft Morphology, Knob Architecture, and P. falciparum E MP1 Trafficking | McHugh E, Carmo OMS, ..., Dixon MWA | 10.1128/mBio.03320-19 |
| 19849836 | Simultaneous transcription of duplicated var2csa gene copies in individualPlasmodium falciparum parasites | Brolin KJ, Ribacke U, ..., Chen Q | 10.1186/gb-2009-10-10-r117 |
| 20653104 | Immunogenicity of the Plasmodium falciparum Pf332-DBL domain in combination withdifferent adjuvants | Du C, Nilsson S, ..., Chen Q | 10.1016/j.vaccine.2010.05.027 |
| 19822232 | Immunogenicity and antigenic properties of Pf332-C231, a fragment of a non-repeatregion of the Plasmodium falciparum antigen Pf332 | Balogun HA, Vasconcelos NM, ..., Berzins K | 10.1016/j.vaccine.2009.09.110 |
| 24200839 | Pattern of antibodies to the Duffy binding like domain of Plasmodium falciparumantigen Pf332 in Senegalese individuals | Balogun HA, Awah N, ..., Berzins K | 10.1016/j.actatropica.2013.10.018 |
| 19284648 | FcgammaRIIa (CD32) polymorphism and anti-malarial IgG subclass pattern amongFulani and sympatric ethnic groups living in eastern Sudan | Nasr A, Iriemenam NC, ..., Berzins K | 10.1186/1475-2875-8-43 |
| 21645028 | Pattern of pre-existing IgG subclass responses to a panel of asexual stagemalaria antigens reported during the lengthy dry season in Daraweesh, Sudan | Nasr A, Iriemenam NC, ..., Elghazali G | 10.1111/j.1365-3083.2011.02585.x |
| 20132790 | Interaction of the exported malaria protein Pf332 with the red blood cellmembrane skeleton | Waller KL, Stubberfield LM, ..., Cooke BM | 10.1016/j.bbamem.2010.01.018 |
| 20215002 | Clustering of malaria treatment failure (TF) in Daraweesh: hints for host geneticsusceptibility to TF with emphasis on immune-modulating SNPs | Giha HA, ElGhazali G, ..., Arnot D | 10.1016/j.meegid.2010.02.013 |
| 20036751 | Age-dependent association between IgG2 and IgG3 subclasses to Pf332-C231 antigenand protection from malaria, and induction of protective antibodies by sub-patentmalaria infections, in Daraweesh | Giha HA, Nasr A, ..., ElGhazali G | 10.1016/j.vaccine.2009.12.018 |
| 37000891 | Formation of ER-lumenal intermediates during export of Plasmodium proteinscontaining transmembrane-like hydrophobic sequences | Levray YS, Bana B, ..., Osborne AR | 10.1371/journal.ppat.1011281 |
| 22744729 | A malaria serological map indicating the intersection between parasite antigenicdiversity and host antibody repertoires | Giha HA, Nasr AA, ..., Elghazali G | 10.1007/s10096-012-1673-z |
| 20028548 | Antigen-specific influence of GM/KM allotypes on IgG isotypes and association ofGM allotypes with susceptibility to Plasmodium falciparum malaria | Giha HA, Nasr A, ..., Pandey JP | 10.1186/1475-2875-8-306 |
| 21729768 | Associations of multi-locus polymorphisms in an immune network withsusceptibility to uncomplicated Plasmodium falciparum malaria in Daraweeshvillage, Eastern Sudan | Giha HA, Nasr A, ..., Elghazali G | 10.1016/j.meegid.2011.06.015 |
| 29100811 | Plasmodium falciparum exported protein PFE60 influences Maurer's cleftsarchitecture and virulence complex composition | Zhang M, Faou P, Maier AG, Rug M | 10.1016/j.ijpara.2017.09.003 |
| 22312570 | Characterization of the Duffy-Binding-Like Domain of Plasmodium falciparumBlood-Stage Antigen 332 | Nilsson S, Moll K, ..., Chen Q | 10.4061/2011/671439 |
| 28320404 | Tryptophan-rich domains of Plasmodium falciparum SURFIN(4.2) and Plasmodium vivaxPvSTP2 interact with membrane skeleton of red blood cell | Zhu X, He Y, ..., Cao Y | 10.1186/s12936-017-1772-5 |
| 22064263 | Pf332-C231-reactive antibodies affect growth and development ofintra-erythrocytic Plasmodium falciparum parasites | Balogun HA, Awah NW, Farouk SE, Berzins K | 10.1016/j.vaccine.2011.10.063 |