PF3D7_1149600

Genome location: Pf3D7_11_v3:2,000,342..2,003,996(+)

Genome classification: Core

Function and Localization

Product Description: DnaJ protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft
Component	GO:0043657	host cell

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.67	0.35
Trophozoite	expressed	0.30	0.84
Schizont	expressed	0.24	0.86
Gametocyte	not expressed	0.13	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1149600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.493 | #Insertions: 4

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1238200)

Relative Growth Rate: 0.78 ± 0.07
Confidence: 6.69

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IHM7

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102152

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	23	33	20	25
disruptive	48	94	54	113
missense	45	92	51	91

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	28	63	21	28
disruptive	70	183	78	165
missense	62	165	70	103

PlasmoDB Total SNPs: 204

Non-coding: 137 | Synonymous: 25 | Nonsynonymous: 41 | Stop Codon: 1

Protein Information

Protein Length: 714 | Molecular Weight (kDa): 83.12

UniProt ID(s): Q8IHM7

PDB ID(s): None

Isoelectric Point: 9

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001623	DnaJ domain
InterPro	IPR018253	DnaJ domain, conserved site
InterPro	IPR026894	DNAJ-containing protein, X-domain
InterPro	IPR036869	Chaperone J-domain superfamily
PFam	PF00226	DnaJ domain
PFam	PF14308	DNAJ-containing protein, X-domain
Superfamily	SSF46565	Chaperone J-domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16507167	Lineage-specific expansion of proteins exported to erythrocytes in malariaparasites.	Sargeant TJ, Marti M, ..., Cowman AF	10.1186/gb-2006-7-2-r12
31631203	A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum.	Das D, Krishnan SR, Roy A, Bulusu G	10.1039/c9mo00124g