Product Description: acyl-CoA synthetase
SignalP Peptide: MNITLSLFFSVIYVVYVLPCCTQLSNS
# Transmembrane Domains: 0
EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0016020 | membrane |
| Function | GO:0004467 | long-chain fatty acid-CoA ligase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 2.42 | 0.22 |
| Trophozoite | expressed | 0.45 | 0.77 |
| Schizont | expressed | 0.44 | 0.76 |
| Gametocyte | not expressed | 0.23 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1200700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.229 | #Insertions: 3
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I638
"Best" AlphaFill ligand hit: COA (coenzyme a, Local RMSD=0.16) with 5BSR (Global RMSD=3.95)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.3 | long-chain-fatty-acid-CoA ligase | ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100152
Most Similar Human Ortholog: B7Z3Z9
TM-align score: 0.90 | RMSD: 2.74
Seq Identity: 0.25 | Length: 516 / 926
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000151726 | acyl-CoA synthetase long chain family member 1 |
| ENSG00000164398 | acyl-CoA synthetase long chain family member 6 |
| ENSG00000197142 | acyl-CoA synthetase long chain family member 5 |
| ENSG00000281938 | novel transcript |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 18 | 11 | 24 |
| disruptive | 31 | 33 | 22 | 81 |
| missense | 28 | 29 | 18 | 61 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 32 | 23 | 41 |
| disruptive | 44 | 121 | 83 | 162 |
| missense | 34 | 77 | 55 | 103 |
PlasmoDB Total SNPs: 158
Non-coding: 77 | Synonymous: 41 | Nonsynonymous: 40 | Stop Codon: 0
Protein Length: 926 | Molecular Weight (kDa): 104.621
UniProt ID(s): Q8I638
PDB ID(s): None
Isoelectric Point: 9.02
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000873 | AMP-dependent synthetase/ligase |
| InterPro | IPR020845 | AMP-binding, conserved site |
| PFam | PF00501 | AMP-dependent synthetase/ligase |
| Superfamily | SSF56801 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |