About Gene List

PF3D7_1200800 (FIKK12)

Genome location: Pf3D7_12_v3:68,555..72,206(-)

Genome classification: Core

Function and Localization

Product Description: serine/threonine protein kinase, FIKK family

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020036 Maurer's cleft
Component GO:0005737 cytoplasm
Component GO:0033644 host cell membrane
Component GO:0005634 nucleus
Function GO:0004674 protein serine/threonine kinase activity
Process GO:0035556 intracellular signal transduction
Process GO:0044003 modulation by symbiont of host process
Process GO:0006468 protein phosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 1.33 0.45
Trophozoite expressed 0.41 0.77
Schizont expressed 0.17 0.90
Gametocyte possibly expressed 0.30 0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1200800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.428 | #Insertions: 3

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1225000)

  • Relative Growth Rate: 0.30 ± 0.11
  • Confidence: 5.77

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I637

"Best" AlphaFill ligand hit: GEN (5,7-dihydroxy-3-(4-hydroxyphenyl)-4h-1-benzopyran-4-one, Local RMSD=1.50) with 5AUZ (Global RMSD=11.34)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.11.1 non-specific serine/threonine protein kinase ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_111291

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 5 12 11 17
disruptive 17 44 30 69
missense 17 44 28 59

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 8 23 18 32
disruptive 28 108 62 128
missense 26 92 52 78

PlasmoDB Total SNPs: 234

Non-coding: 211 | Synonymous: 11 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 567 | Molecular Weight (kDa): 67.531

UniProt ID(s): Q8I637

PDB ID(s): None

Isoelectric Point: 8.47

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000719 Protein kinase domain
InterPro IPR008266 Tyrosine-protein kinase, active site
InterPro IPR011009 Protein kinase-like domain superfamily
PFam PF00069 Protein kinase domain
Superfamily SSF56112 Protein kinase-like domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
17181785 A novel protein kinase family in Plasmodium falciparum is differentiallytranscribed and secreted to various cellular compartments of the host cell. Nunes MC, Goldring JP, Doerig C, Scherf A 10.1111/j.1365-2958.2006.05521.x
20668526 Plasmodium falciparum FIKK kinase members target distinct components of theerythrocyte membrane. Nunes MC, Okada M, ..., Scherf A 10.1371/journal.pone.0011747
32284562 An exported kinase family mediates species-specific erythrocyte remodelling andvirulence in human malaria. Davies H, Belda H, ..., Treeck M 10.1038/s41564-020-0702-4