Product Description: NIMA related kinase 3
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0004708 | MAP kinase kinase activity |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0046777 | protein autophosphorylation |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | not expressed | 0.12 | 0.93 |
| Schizont | not expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 1.24 | 0.50 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1201600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.643 | MFS: -3.088 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I629
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.08) with 6P7G (Global RMSD=6.27)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 12 | 6 | 17 |
| disruptive | 9 | 16 | 9 | 17 |
| missense | 8 | 14 | 8 | 15 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 24 | 10 | 20 |
| disruptive | 11 | 36 | 9 | 64 |
| missense | 10 | 28 | 6 | 40 |
PlasmoDB Total SNPs: 46
Non-coding: 27 | Synonymous: 10 | Nonsynonymous: 9 | Stop Codon: 0
Protein Length: 347 | Molecular Weight (kDa): 40.431
UniProt ID(s): Q8I629
PDB ID(s): None
Isoelectric Point: 6.09
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 17064692 | Pfnek3: an atypical activator of a MAP kinase in Plasmodium falciparum. | Lye YM, Chan M, Sim TS | 10.1016/j.febslet.2006.10.003 |
| 19644735 | Regulation of Plasmodium falciparum Pfnek3 relies on phosphorylation at itsactivation loop and at threonine 82. | Low H, Chua CS, Sim TS | 10.1007/s00018-009-0101-8 |
| 22116321 | Plasmodium falciparum possesses a unique dual-specificity serine/threonine andtyrosine kinase, Pfnek3. | Low H, Chua CS, Sim TS | 10.1007/s00018-011-0888-y |