Product Description: ATP-dependent DNA/RNA helicase PSH2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase);3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0043138 | 3'-5' DNA helicase activity |
| Function | GO:0043139 | 5'-3' DNA helicase activity |
| Function | GO:0005524 | ATP binding |
| Function | GO:0008094 | ATP-dependent activity, acting on DNA |
| Function | GO:0008186 | ATP-dependent activity, acting on RNA |
| Function | GO:0003724 | RNA helicase activity |
| Process | GO:0032508 | DNA duplex unwinding |
| Process | GO:0006364 | rRNA processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.27 | 0.87 |
| Trophozoite | expressed | 0.59 | 0.67 |
| Schizont | expressed | 0.16 | 0.90 |
| Gametocyte | possibly expressed | 0.68 | 0.63 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1202000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -2.992 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0601000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A0Z6
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.03) with 6L5M (Global RMSD=7.53)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 35 | 16 | 38 |
| disruptive | 19 | 87 | 49 | 103 |
| missense | 18 | 86 | 49 | 91 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 61 | 24 | 53 |
| disruptive | 49 | 182 | 71 | 182 |
| missense | 37 | 159 | 60 | 148 |
PlasmoDB Total SNPs: 186
Non-coding: 116 | Synonymous: 40 | Nonsynonymous: 30 | Stop Codon: 0
Protein Length: 880 | Molecular Weight (kDa): 105.02
UniProt ID(s): A0A144A0Z6, A0A386KSN1
PDB ID(s): None
Isoelectric Point: 9.55
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30728406 | Plasmodium falciparum specific helicase 2 is a dual, bipolar helicase and iscrucial for parasite growth. | Chauhan M, Tuteja R | 10.1038/s41598-018-38032-1 |