PF3D7_1202500 (TGS1)

Genome location: Pf3D7_12_v3:144,046..148,030(-)

Genome classification: Core

Function and Localization

Product Description: trimethylguanosine synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0008173	RNA methyltransferase activity
Function	GO:0071164	RNA trimethylguanosine synthase activity
Process	GO:0036261	7-methylguanosine cap hypermethylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.38	0.81
Trophozoite	possibly expressed	0.29	0.84
Schizont	not expressed	0.18	0.88
Gametocyte	not expressed	0.34	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1202500

Resistome Missense Mutations: D104N

Resistome Compounds with Missense Mutations: BI-2536

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.982 | MFS: -2.691 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0601500)

Relative Growth Rate: 0.98 ± 0.18
Confidence: 4.79

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0601500)

Modification: Disrupted | RMgm-2055

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A060

"Best" AlphaFill ligand hit: MGP (7-methyl-guanosine-5'-triphosphate, Local RMSD=0.56) with 3GDH (Global RMSD=2.71)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102135

Most Similar Human Ortholog: E5RJW7

TM-align score: 0.42 | RMSD: 3.42

Seq Identity: 0.04 | Length: 48 / 1079

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000137574	trimethylguanosine synthase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	71	28	89
disruptive	38	190	127	208
missense	30	184	122	186

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	26	114	57	78
disruptive	81	402	143	278
missense	58	364	130	215

PlasmoDB Total SNPs: 170

Non-coding: 54 | Synonymous: 76 | Nonsynonymous: 40 | Stop Codon: 0

Protein Information

Protein Length: 1079 | Molecular Weight (kDa): 131.986

UniProt ID(s): A0A144A060

PDB ID(s): None

Isoelectric Point: 9.72

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR019012	RNA cap guanine-N2 methyltransferase
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF09445	RNA cap guanine-N2 methyltransferase
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase