Product Description: threonylcarbamoyl-AMP synthase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.87 (L-threonylcarbamoyladenylate synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0016779 | nucleotidyltransferase activity |
| Function | GO:0000049 | tRNA binding |
| Process | GO:0006450 | regulation of translational fidelity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.71 | 0.67 |
| Trophozoite | possibly expressed | 0.56 | 0.68 |
| Schizont | expressed | 0.13 | 0.92 |
| Gametocyte | possibly expressed | 0.48 | 0.72 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1203500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.128 | MFS: -2.983 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I610
"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.40) with 6F87 (Global RMSD=2.82)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.87 | L-threonylcarbamoyladenylate synthase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100577
Most Similar Human Ortholog: Q86U90
TM-align score: 0.71 | RMSD: 2.32
Seq Identity: 0.20 | Length: 220 / 556
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000196449 | yrdC N6-threonylcarbamoyltransferase domain containing |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 42 | 13 | 50 |
| disruptive | 18 | 81 | 50 | 105 |
| missense | 12 | 72 | 47 | 86 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 75 | 31 | 46 |
| disruptive | 40 | 175 | 98 | 205 |
| missense | 30 | 148 | 64 | 127 |
PlasmoDB Total SNPs: 90
Non-coding: 47 | Synonymous: 27 | Nonsynonymous: 16 | Stop Codon: 0
Protein Length: 556 | Molecular Weight (kDa): 64.888
UniProt ID(s): Q8I610
PDB ID(s): None
Isoelectric Point: 5.5
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005145 | Putative GTP-binding controlling metal-binding domain |
| InterPro | IPR006070 | YrdC-like domain |
| InterPro | IPR017945 | DHBP synthase RibB-like alpha/beta domain superfamily |
| PFam | PF01300 | YrdC-like domain |
| PFam | PF03481 | Putative GTP-binding controlling metal-binding domain |
| Superfamily | SSF55821 | DHBP synthase RibB-like alpha/beta domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 25204654 | Kinase-associated endopeptidase 1 (Kae1) participates in an atypicalribosome-associated complex in the apicoplast of Plasmodium falciparum. | Mallari JP, Oksman A, Vaupel B, Goldberg DE | 10.1074/jbc.M114.586735 |
| 36362385 | Conservation and Diversification of tRNA t(6)A-Modifying Enzymes across the ThreeDomains of Life | Su C, Jin M, Zhang W | 10.3390/ijms232113600 |
| 35477969 | Establishment and a comparative transcriptomic analysis of a male-specific cellline from the African malaria mosquito Anopheles gambiae | Krzywinska E, Ferretti L, Krzywinski J | 10.1038/s41598-022-10686-y |
| 25038083 | Cross kingdom functional conservation of the core universally conserved threonylcarbamoyladenosine tRNA synthesis enzymes | Thiaville PC, El Yacoubi B, ..., de Crecy-Lagard V | 10.1128/EC.00147-14 |
| 29804714 | Yeast KEOPS complex regulates telomere length independently of its t(6)Amodification function | Liu YY, He MH, ..., Zhou JQ | 10.1016/j.jgg.2018.03.004 |
| 37427786 | Structure-function analysis of an ancient TsaD-TsaC-SUA5-TcdA modular enzyme reveals a prototype of tRNA t6A and ct6A synthetases | Jin M, Zhang Z, ..., Zhang W | 10.1093/nar/gkad587 |
| 20309016 | Sua5p is required for telomere recombination in Saccharomyces cerevisiae | Meng FL, Chen XF, ..., Zhou JQ | 10.1038/cr.2010.34 |
| 38085018 | Sua5 catalyzing universal t(6)A tRNA modification is responsible for multifacetedfunctions of the KEOPS complex in Cryptococcus neoformans | Choi Y, Hyeon H, Lee K, Bahn Y-S | 10.1128/msphere.00557-23 |
| 23620299 | Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system | Wan LC, Mao DY, ..., Sicheri F | 10.1093/nar/gkt322 |
| 23258706 | In vitro biosynthesis of a universal t6A tRNA modification in Archaea andEukarya | Perrochia L, Crozat E, ..., Basta T | 10.1093/nar/gks1287 |
| 32085421 | Loss of Elongator- and KEOPS-Dependent tRNA Modifications Leads to Severe GrowthPhenotypes and Protein Aggregation in Yeast | Pollo-Oliveira L, Klassen R, ..., de Crecy-Lagard V | 10.3390/biom10020322 |
| 21538543 | Crystal structure of Sulfolobus tokodaii Sua5 complexed with L-threonine andAMPPNP | Kuratani M, Kasai T, ..., Yokoyama S | 10.1002/prot.23026 |
| 37415821 | The universal Sua5/TsaC family evolved different mechanisms for the synthesis of a key tRNA modification | Pichard-Kostuch A, Da Cunha V, ..., Basta T | 10.3389/fmicb.2023.1204045 |
| 19884342 | The Sua5 protein is essential for normal translational regulation in yeast | Lin CA, Ellis SR, True HL | 10.1128/MCB.00754-09 |
| 29650678 | Structure-function analysis of Sua5 protein reveals novel functional motifs required for the biosynthesis of the universal t(6)A tRNA modification | Pichard-Kostuch A, Zhang W, ..., Basta T | 10.1261/rna.066092.118 |