Product Description: cytochrome c1 heme lyase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.4.1.17 (Holocytochrome-c synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005743 | mitochondrial inner membrane |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004408 | holocytochrome-c synthase activity |
| Process | GO:0018063 | cytochrome c-heme linkage |
| Process | GO:0055114 | oxidation-reduction process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.13 | 0.91 |
| Schizont | expressed | 0.04 | 0.97 |
| Gametocyte | expressed | 0.14 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1203600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.029 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1439500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I609
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.4.1.17 | Holocytochrome-c synthase | heminDeuteroheminN-ethylmaleimideHolocytochrome c |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101849
Most Similar Human Ortholog: P53701
TM-align score: 0.73 | RMSD: 2.64
Seq Identity: 0.37 | Length: 153 / 184
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000004961 | holocytochrome c synthase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 6 | 3 | 13 |
| disruptive | 0 | 15 | 11 | 14 |
| missense | 0 | 15 | 10 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 21 | 4 | 21 |
| disruptive | 7 | 40 | 22 | 58 |
| missense | 2 | 33 | 16 | 40 |
PlasmoDB Total SNPs: 102
Non-coding: 90 | Synonymous: 9 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 184 | Molecular Weight (kDa): 21.846
UniProt ID(s): Q8I609
PDB ID(s): None
Isoelectric Point: 9.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000511 | Cytochrome c/c1 haem-lyase |
| PFam | PF01265 | Cytochrome c/c1 haem-lyase |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 20697122 | c-type cytochrome assembly in Saccharomyces cerevisiae: a key residue forapocytochrome c1/lyase interaction | Corvest V, Murrey DA, ..., Hamel PP | 10.1534/genetics.110.120022 |
| 16207709 | Cyc2p, a membrane-bound flavoprotein involved in the maturation of mitochondrialc-type cytochromes | Bernard DG, Quevillon-Cheruel S, ..., Hamel PP | 10.1074/jbc.M508574200 |
| 8955088 | Heme binding to a conserved Cys-Pro-Val motif is crucial for the catalytic function of mitochondrial heme lyases | Steiner H, Kispal G, ..., Lill R | 10.1074/jbc.271.51.32605 |
| 22732413 | Engineering a prokaryotic apocytochrome c as an efficient substrate forSaccharomyces cerevisiae cytochrome c heme lyase | Verissimo AF, Sanders J, Daldal F, Sanders C | 10.1016/j.bbrc.2012.06.088 |
| 14514677 | Overlapping specificities of the mitochondrial cytochrome c and c1 heme lyases | Bernard DG, Gabilly ST, ..., Hamel PP | 10.1074/jbc.M308881200 |