Product Description: ubiquitin-conjugating enzyme E2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.2.23 (E2 ubiquitin-conjugating enzyme)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0061631 | ubiquitin conjugating enzyme activity |
| Function | GO:0004842 | ubiquitin-protein transferase activity |
| Process | GO:0006464 | cellular protein modification process |
| Process | GO:0000209 | protein polyubiquitination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.19 | 0.90 |
| Trophozoite | expressed | 0.22 | 0.86 |
| Schizont | expressed | 0.25 | 0.83 |
| Gametocyte | expressed | 0.34 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1203900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.554 | MFS: -2.159 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0602800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I607
"Best" AlphaFill ligand hit: BU3 ((r,r)-2,3-butanediol, Local RMSD=0.05) with 1Z2U (Global RMSD=0.77)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.2.23 | E2 ubiquitin-conjugating enzyme | CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P63146 | Ubiquitin-conjugating enzyme E2 B | OrthoDB | CHEMBL3787660 | |
| P51965 | Ubiquitin-conjugating enzyme E2 E1 | OrthoDB | US9796725, Compound I-001US9796725, Compound I-098US9796725, Compound I-003US9796725, Compound I-004... | |
| P61088 | Ubiquitin-conjugating enzyme E2 N | Homo sapiens | OrthoDB | MLS000074439MLS000032400MLS000041097MLS000055488... |
Ortholog Group (OrthoMCL): OG6_100671
Most Similar Human Ortholog: P62837
TM-align score: 1.00 | RMSD: 0.19
Seq Identity: 0.76 | Length: 147 / 147
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000072401 | ubiquitin conjugating enzyme E2 D1 |
| ENSG00000078967 | ubiquitin conjugating enzyme E2 D4 (putative) |
| ENSG00000109332 | ubiquitin conjugating enzyme E2 D3 |
| ENSG00000131508 | ubiquitin conjugating enzyme E2 D2 |
Protein Length: 147 | Molecular Weight (kDa): 16.539
UniProt ID(s): Q8I607
PDB ID(s): None
Isoelectric Point: 7.24
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000608 | Ubiquitin-conjugating enzyme E2 |
| InterPro | IPR016135 | Ubiquitin-conjugating enzyme/RWD-like |
| InterPro | IPR023313 | Ubiquitin-conjugating enzyme, active site |
| PFam | PF00179 | Ubiquitin-conjugating enzyme E2 |
| Superfamily | SSF54495 | Ubiquitin-conjugating enzyme/RWD-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 7 | 6 | 10 |
| disruptive | 0 | 0 | 0 | 5 |
| missense | 0 | 0 | 0 | 2 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 18 | 5 | 12 |
| disruptive | 0 | 7 | 13 | 32 |
| missense | 0 | 3 | 8 | 24 |
PlasmoDB Total SNPs: 123
Non-coding: 121 | Synonymous: 2 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 22912882 | Characterization of the ubiquitylating components of the human malaria parasite'sprotein degradation pathway. | Chung DW, Ponts N, ..., Le Roch KG | 10.1371/journal.pone.0043477 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |