PF3D7_1204600

Genome location: Pf3D7_12_v3:212,829..214,204(+)

Genome classification: Core

Function and Localization

Product Description: cytidine deaminase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.5.4.5 (Cytidine deaminase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0052718	tRNA-specific adenosine-34 deaminase complex
Function	GO:0052717	tRNA-specific adenosine-34 deaminase activity
Process	GO:0002100	tRNA wobble adenosine to inosine editing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.14	0.93
Trophozoite	expressed	0.19	0.88
Schizont	expressed	0.05	0.97
Gametocyte	expressed	0.16	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1204600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.142 | MFS: -2.665 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0603500)

Relative Growth Rate: 0.17 ± 0.24
Confidence: 4.22

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5Z9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.5.4.5	cytidine deaminase	NH3GTPCTPUTPCMPSDSCDPEDTAdTTPdUTPdCMPDTNB...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103273

Most Similar Human Ortholog: D6W601

TM-align score: 0.74 | RMSD: 2.69

Seq Identity: 0.24 | Length: 268 / 326

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000213638	adenosine deaminase tRNA specific 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	10	12	17
disruptive	6	43	22	59
missense	5	39	20	52

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	29	10	18
disruptive	19	88	40	83
missense	14	78	30	66

PlasmoDB Total SNPs: 58

Non-coding: 28 | Synonymous: 20 | Nonsynonymous: 8 | Stop Codon: 2

Protein Information

Protein Length: 326 | Molecular Weight (kDa): 38.564

UniProt ID(s): Q8I5Z9

PDB ID(s): None

Isoelectric Point: 8.77

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002125	Cytidine and deoxycytidylate deaminase domain
InterPro	IPR016193	Cytidine deaminase-like
PFam	PF00383	Cytidine and deoxycytidylate deaminase domain
Superfamily	SSF53927	Cytidine deaminase-like