Genome location: Pf3D7_12_v3:222,428..225,423(-)
Genome classification: Core
Product Description: O-phosphoseryl-tRNA(Sec) selenium transferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.9.1.2 (O-phospho-L-seryl-tRNA(Sec):L-selenocysteinyl-tRNA synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0000049 | tRNA binding |
| Process | GO:0001514 | selenocysteine incorporation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.07 | 0.96 |
| Trophozoite | expressed | 0.25 | 0.85 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | expressed | 0.16 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1205100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.306 | #Insertions: 5
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0603800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0603800)
Modification: Disrupted | RMgm-2064
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5Z4
"Best" AlphaFill ligand hit: PLR (4'-deoxypyridoxine phosphate, Local RMSD=0.42) with 3HL2 (Global RMSD=2.89)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.9.1.2 | O-phospho-L-seryl-tRNASec:L-selenocysteinyl-tRNA synthase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_104780
Most Similar Human Ortholog: A0A7P0Z4P3
TM-align score: 0.96 | RMSD: 1.49
Seq Identity: 0.36 | Length: 340 / 578
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000109618 | Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 43 | 17 | 43 |
| disruptive | 10 | 56 | 43 | 80 |
| missense | 10 | 56 | 41 | 71 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 72 | 32 | 55 |
| disruptive | 15 | 129 | 61 | 154 |
| missense | 14 | 113 | 53 | 125 |
PlasmoDB Total SNPs: 113
Non-coding: 64 | Synonymous: 30 | Nonsynonymous: 19 | Stop Codon: 0
Protein Length: 578 | Molecular Weight (kDa): 66.815
UniProt ID(s): Q8I5Z4
PDB ID(s): None
Isoelectric Point: 8.69
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR008829 | SepSecS/SepCysS family |
| InterPro | IPR015424 | Pyridoxal phosphate-dependent transferase |
| InterPro | IPR019872 | O-phosphoseryl-tRNA(Sec) selenium transferase |
| PFam | PF05889 | SepSecS/SepCysS family |
| Superfamily | SSF53383 | Pyridoxal phosphate-dependent transferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34476677 | Selenium-enriched Polysaccharide: an Effective and Safe Selenium Source of C57Mice to Improve Growth Performance, Regulate Selenium Deposition, and PromoteAntioxidant Capacity | Cao J, Liu X, ..., Wang F | 10.1007/s12011-021-02832-w |