Product Description: targeted glyoxalase II
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.2.6 (Hydroxyacylglutathione hydrolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004416 | hydroxyacylglutathione hydrolase activity |
| Process | GO:0009438 | methylglyoxal metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.12 | 0.92 |
| Schizont | expressed | 0.11 | 0.92 |
| Gametocyte | expressed | 0.77 | 0.61 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1205700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.692 | #Insertions: 3
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0604400)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0604400)
Modification: Disrupted | RMgm-4977
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5Y8
"Best" AlphaFill ligand hit: LAC (lactic acid, Local RMSD=0.19) with 2P1E (Global RMSD=4.82)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.2.6 | hydroxyacylglutathione hydrolase | ATPADPAMPGTPGDPCTPUTPKClNEMCMPUMPGSH... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q3B7M2 | Hydroxyacylglutathione hydrolase, mitochondrial | OrthoDB | CHEMBL3629116CHEMBL3629115CHEMBL131578 | |
| O35952 | Hydroxyacylglutathione hydrolase, mitochondrial | Rattus norvegicus | OrthoDB | CHEMBL355031CHEMBL168630 |
| Q16775 | Hydroxyacylglutathione hydrolase, mitochondrial | Homo sapiens | OrthoDB | S-(N-Hydroxy-N-(4-chlorophenyl)carbamoyl)glutathioneS-(N-Hydroxy-N-(4-bromophenyl)carbamoyl)glutathione |
Ortholog Group (OrthoMCL): OG6_100757
Most Similar Human Ortholog: O95571
TM-align score: 0.70 | RMSD: 2.83
Seq Identity: 0.19 | Length: 204 / 322
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000105755 | ETHE1 persulfide dioxygenase |
Protein Length: 322 | Molecular Weight (kDa): 38.414
PDB ID(s): None
Isoelectric Point: 8.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001279 | Metallo-beta-lactamase |
| InterPro | IPR032282 | Hydroxyacylglutathione hydrolase, C-terminal domain |
| InterPro | IPR035680 | Hydroxyacylglutathione hydrolase, MBL domain |
| InterPro | IPR036866 | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
| PFam | PF00753 | Metallo-beta-lactamase |
| PFam | PF16123 | Hydroxyacylglutathione hydrolase, C-terminal domain |
| Superfamily | SSF56281 | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 18 | 7 | 14 |
| disruptive | 2 | 18 | 15 | 58 |
| missense | 2 | 17 | 15 | 54 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 36 | 13 | 18 |
| disruptive | 5 | 53 | 43 | 92 |
| missense | 2 | 50 | 37 | 71 |
PlasmoDB Total SNPs: 70
Non-coding: 63 | Synonymous: 4 | Nonsynonymous: 3 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15843146 | Characterization of the glyoxalases of the malarial parasite Plasmodiumfalciparum and comparison with their human counterparts. | Akoachere M, Iozef R, ..., Becker K | 10.1515/BC.2005.006 |
| 20149108 | Distinct subcellular localization in the cytosol and apicoplast, unexpecteddimerization and inhibition of Plasmodium falciparum glyoxalases. | Urscher M, Przyborski JM, Imoto M, Deponte M | 10.1111/j.1365-2958.2010.07082.x |
| 21203490 | Compartmentation of redox metabolism in malaria parasites. | Kehr S, Sturm N, ..., Becker K | 10.1371/journal.ppat.1001242 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |