Product Description: IMP-specific 5'-nucleotidase 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.5 (5'-nucleotidase);3.1.3.99 (IMP-specific 5'-nucleotidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Function | GO:0005524 | ATP binding |
| Function | GO:0050483 | IMP 5'-nucleotidase activity |
| Process | GO:0016311 | dephosphorylation |
| Process | GO:0006190 | inosine salvage |
| Process | GO:0071590 | nicotinamide riboside biosynthetic process |
| Process | GO:0071592 | nicotinic acid riboside biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | not expressed | 0.04 | 0.98 |
| Schizont | possibly expressed | 0.13 | 0.91 |
| Gametocyte | expressed | 0.34 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1206100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.991 | MFS: -0.085 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A134
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.27) with 6RMD (Global RMSD=1.55)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 21 | 16 | 20 |
| disruptive | 0 | 7 | 10 | 23 |
| missense | 0 | 7 | 9 | 19 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 41 | 14 | 28 |
| disruptive | 2 | 29 | 21 | 58 |
| missense | 0 | 19 | 19 | 41 |
PlasmoDB Total SNPs: 142
Non-coding: 117 | Synonymous: 23 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 444 | Molecular Weight (kDa): 51.907
UniProt ID(s): A0A144A134
PDB ID(s): 6RMD, 6RME, 6RMO, 6RMW, 6RN1, 6RNH
Isoelectric Point: 7.17
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR009453 | IMP-specific 5-nucleotidase |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF06437 | IMP-specific 5-nucleotidase |
| Superfamily | SSF56784 | HAD-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 32591529 | Structure and catalytic regulation of Plasmodium falciparum IMP specificnucleotidase. | Carrique L, Ballut L, ..., Aghajari N | 10.1038/s41467-020-17013-x |
| 37402342 | Purine containing carbonucleoside phosphonate analogues as novel chemotype forPlasmodium falciparum Inhibition | Mohamed BS, Nguyen MC, ..., Peyrottes S | 10.1016/j.ejmech.2023.115581 |
| 37569166 | Enhanced Production of beta-Nicotinamide Mononucleotide with Exogenous NicotinamideAddition in Saccharomyces boulardii-YS01 | Song M, Yin C, ..., Yan H | 10.3390/foods12152897 |
| 38362806 | Structure, cooperativity and inhibition of the inosine 5'-monophosphate-specificphosphatase from Saccharomyces cerevisiae | Byun S, Park C, ..., Rhee S | 10.1111/febs.17093 |
| 12735798 | The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5'-nucleotidase | Itoh R, Saint-Marc C, ..., Daignan-Fornier B | 10.1186/1471-2091-4-4 |
| 17192850 | Revised procedures for yeast metabolites extraction: application to a glucosepulse to carbon-limited yeast cultures, which reveals a transient activation ofthe purine salvage pathway | Loret MO, Pedersen L, Francois J | 10.1002/yea.1435 |
| 20087341 | Control of ATP homeostasis during the respiro-fermentative transition in yeast | Walther T, Novo M, ..., Francois JM | 10.1038/msb.2009.100 |