Product Description: thiosulfate sulfurtransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.8.1.1 (Thiosulfate sulfurtransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004792 | thiosulfate sulfurtransferase activity |
| Process | GO:0002098 | tRNA wobble uridine modification |
| Process | GO:0019346 | transsulfuration |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 1.00 |
| Trophozoite | not expressed | 0.03 | 0.98 |
| Schizont | possibly expressed | 0.19 | 0.87 |
| Gametocyte | not expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1206400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.999 | MFS: -0.46 | #Insertions: 2
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5Y1
"Best" AlphaFill ligand hit: PYR (pyruvic acid, Local RMSD=4.65) with 4JGT (Global RMSD=4.39)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.8.1.1 | thiosulfate sulfurtransferase | NEMSDSH2SCaCl2SO42-SO32-malateanionssulfitecitrateSulfidelipoate... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q16762 | Thiosulfate sulfurtransferase | OrthoDB | Beta lapachoneHARMALOLmethylthioninium chlorideRiboflavin sodium phosphate... |
Protein Length: 346 | Molecular Weight (kDa): 41.929
UniProt ID(s): Q8I5Y1
PDB ID(s): None
Isoelectric Point: 7.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001763 | Rhodanese-like domain |
| InterPro | IPR036873 | Rhodanese-like domain superfamily |
| PFam | PF00581 | Rhodanese-like domain |
| Superfamily | SSF52821 | Rhodanese-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 16 | 4 | 21 |
| disruptive | 1 | 26 | 21 | 71 |
| missense | 1 | 25 | 20 | 63 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 34 | 10 | 25 |
| disruptive | 9 | 71 | 35 | 90 |
| missense | 5 | 59 | 28 | 68 |
PlasmoDB Total SNPs: 55
Non-coding: 47 | Synonymous: 6 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21821903 | Crystallization and preliminary X-ray analysis of the yeast tRNA-thiouridinemodification protein 1 (Tum1p) | Qiu R, Wang F, ..., Ji C | 10.1107/S1744309111024900 |
| 28357324 | Sulfur transfer and activation by ubiquitin-like modifier system Uba4*Urm1 linkprotein urmylation and tRNA thiolation in yeast | Judes A, Bruch A, ..., Schaffrath R | 10.15698/mic2016.11.539 |
| 31240288 | Iterative synthetic strategies and gene deletant experiments enable the firstidentification of polysulfides in Saccharomyces cerevisiae | Pilkington LI, Deed RC, ..., Fedrizzi B | 10.1039/c9cc03020d |
| 22587783 | Crystal structure of the Tum1 protein from the yeast Saccharomyces cerevisiae | Qiu R, Wang F, ..., Ji C | 10.2174/092986612803217060 |
| 27915245 | The yeast TUM1 affects production of hydrogen sulfide from cysteine treatmentduring fermentation | Huang CW, Walker ME, ..., Jiranek V | 10.1093/femsyr/fow100 |
| 28830344 | Tum1 is involved in the metabolism of sterol esters in Saccharomyces cerevisiae | Ursic K, Ogrizovic M, ..., Petrovic U | 10.1186/s12866-017-1088-1 |
| 33001274 | Identification and characterization of a sulfite reductase gene and new insightsregarding the sulfur-containing amino acid metabolism in the basidiomycetousyeast Cryptococcus neoformans | Nguyen PT, Toh-E A, ..., Shimizu K | 10.1007/s00294-020-01112-9 |