Product Description: tRNA dimethylallyltransferase, putative
SignalP Peptide: MPFNKYVLLIYFILLVNINLFKK
# Transmembrane Domains: 0
EC Numbers: 2.5.1.75 (tRNA dimethylallyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0005524 | ATP binding |
| Function | GO:0000049 | tRNA binding |
| Function | GO:0052381 | tRNA dimethylallyltransferase activity |
| Process | GO:0006400 | tRNA modification |
| Process | GO:0008033 | tRNA processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.49 | 0.78 |
| Trophozoite | expressed | 0.82 | 0.58 |
| Schizont | expressed | 0.08 | 0.95 |
| Gametocyte | expressed | 0.39 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1207600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.715 | MFS: -3.103 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0606100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0606100)
Modification: Disrupted | RMgm-2076
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I0X4
"Best" AlphaFill ligand hit: DMA (dimethylallyl diphosphate, Local RMSD=0.93) with 3EPL (Global RMSD=6.98)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.5.1.75 | tRNA dimethylallyltransferase | ATPADPEDTAdiphosphateiodoacetamidep-mercuribenzoatehigh ionic strength |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100923
Most Similar Human Ortholog: S4R2Z0
TM-align score: 0.80 | RMSD: 3.27
Seq Identity: 0.31 | Length: 315 / 601
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000043514 | tRNA isopentenyltransferase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 36 | 23 | 34 |
| disruptive | 10 | 67 | 36 | 116 |
| missense | 9 | 67 | 34 | 102 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 70 | 30 | 44 |
| disruptive | 16 | 146 | 81 | 138 |
| missense | 12 | 136 | 70 | 109 |
PlasmoDB Total SNPs: 103
Non-coding: 66 | Synonymous: 27 | Nonsynonymous: 10 | Stop Codon: 0
Protein Length: 601 | Molecular Weight (kDa): 71.549
UniProt ID(s): Q8I0X4
PDB ID(s): None
Isoelectric Point: 9.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR018022 | IPP transferase |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR039657 | Dimethylallyltransferase |
| PFam | PF01715 | N/A |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 35257658 | Critical role for isoprenoids in apicoplast biogenesis by malaria parasites | Okada M, Rajaram K, ..., Sigala PA | 10.7554/eLife.73208 |