PF3D7_1209400 (CIA1)

Genome location: Pf3D7_12_v3:435,583..439,379(+)

Genome classification: Core

Function and Localization

Product Description: cytosolic iron-sulfur protein assembly protein 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0097361	CIA complex
Process	GO:0016226	iron-sulfur cluster assembly
Process	GO:0097428	protein maturation by iron-sulfur cluster transfer

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.27	0.89
Trophozoite	expressed	0.18	0.89
Schizont	expressed	0.35	0.85
Gametocyte	expressed	0.18	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1209400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.502 | MFS: -2.659 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0607800)

Relative Growth Rate: 0.04 ± 0.05
Confidence: 7.55

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5V5

"Best" AlphaFill ligand hit: ALQ (2-methyl-propionic acid, Local RMSD=3.79) with 4GM8 (Global RMSD=11.34)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102166

Most Similar Human Ortholog: O76071

TM-align score: 0.91 | RMSD: 1.63

Seq Identity: 0.34 | Length: 320 / 792

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000144021	cytosolic iron-sulfur assembly component 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	41	18	57
disruptive	42	107	62	134
missense	24	81	51	103

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	84	24	46
disruptive	59	214	92	178
missense	32	170	77	119

PlasmoDB Total SNPs: 235

Non-coding: 133 | Synonymous: 44 | Nonsynonymous: 58 | Stop Codon: 0

Protein Information

Protein Length: 792 | Molecular Weight (kDa): 91.851

UniProt ID(s): Q8I5V5

PDB ID(s): None

Isoelectric Point: 6.41

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR028608	Probable cytosolic iron-sulfur protein assembly protein, CIAO1/Cia1
InterPro	IPR036322	WD40-repeat-containing domain superfamily
PFam	PF00400	WD40 repeat
Superfamily	SSF50978	WD40-repeat-containing domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
29967244	Histone Chaperone Asf1 Is Required for the Establishment of Repressive Chromatinin Schizosaccharomyces pombe fbp1 Gene Repression	Umeda M, Tsunekawa C, ..., Hirota K	10.1128/MCB.00194-18
23089178	Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast	Tanae K, Horiuchi T, ..., Kawamukai M	10.1016/j.febslet.2012.10.020
22291963	Histone chaperone Asf1 plays an essential role in maintaining genomic stabilityin fission yeast	Tanae K, Horiuchi T, ..., Kawamukai M	10.1371/journal.pone.0030472