PF3D7_1210400

Genome location: Pf3D7_12_v3:466,191..469,771(-)

Genome classification: Core

Function and Localization

Product Description: general transcription factor 3C polypeptide 5, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000127	transcription factor TFIIIC complex

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.46	0.77
Trophozoite	possibly expressed	0.32	0.80
Schizont	possibly expressed	0.08	0.95
Gametocyte	not expressed	0.25	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1210400

Resistome Missense Mutations: I217L

Resistome Compounds with Missense Mutations: OSM-S-106

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.432 | MFS: -2.517 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0608900)

Relative Growth Rate: 0.14 ± 0.21
Confidence: 4.48

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5U5

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103019

Most Similar Human Ortholog: H7BY84

TM-align score: 0.55 | RMSD: 3.64

Seq Identity: 0.24 | Length: 219 / 901

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000148308	general transcription factor IIIC subunit 5

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	56	28	63
disruptive	27	143	75	178
missense	22	133	70	160

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	25	96	41	63
disruptive	58	306	123	233
missense	42	268	108	170

PlasmoDB Total SNPs: 143

Non-coding: 52 | Synonymous: 52 | Nonsynonymous: 39 | Stop Codon: 0

Protein Information

Protein Length: 901 | Molecular Weight (kDa): 107.048

UniProt ID(s): Q8I5U5

PDB ID(s): None

Isoelectric Point: 8.34

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR019136	Transcription factor IIIC subunit 5, HTH domain
InterPro	IPR040454	Transcription factor IIIC subunit Tfc1/Sfc1
InterPro	IPR041499	Transcription factor IIIC subunit Tfc1/Sfc1, triple barrel domain
PFam	PF09734	Transcription factor IIIC subunit 5, HTH domain
PFam	PF17682	Transcription factor IIIC subunit Tfc1/Sfc1, triple barrel domain
Superfamily	N/A	N/A