PF3D7_1210900 (PIGM)

Genome location: Pf3D7_12_v3:480,479..483,033(+)

Genome classification: Core

Function and Localization

Product Description: GPI mannosyltransferase 1

SignalP Peptide: N/A

# Transmembrane Domains: 11

EC Numbers: 2.4.1.- (Hexosyltransferases.);2.4.1.83 (Dolichyl-phosphate beta-D-mannosyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005783	endoplasmic reticulum
Component	GO:1990529	glycosylphosphatidylinositol-mannosyltransferase I complex
Component	GO:0016020	membrane
Function	GO:0000030	mannosyltransferase activity
Process	GO:0006506	GPI anchor biosynthetic process
Process	GO:0006486	protein glycosylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.03	0.99
Trophozoite	expressed	0.14	0.91
Schizont	expressed	0.06	0.95
Gametocyte	expressed	0.26	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1210900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.181 | MFS: -2.588 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0609300)

Relative Growth Rate: 0.17 ± 0.13
Confidence: 5.48

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5U1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.4.1.-	Ex: levansucrase	KIATPADPUDPAMPGTPGDPCTPUTPKClNEMIMP...
2.4.1.83	dolichyl-phosphate beta-D-mannosyltransferase	ATPADPUDPAMPGTPGDPUTPNEMUMPGMPHgCl2CuSO4...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102429

Most Similar Human Ortholog: Q9H3S5

TM-align score: 0.89 | RMSD: 2.57

Seq Identity: 0.35 | Length: 402 / 441

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000143315	phosphatidylinositol glycan anchor biosynthesis class M

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	23	20	27
disruptive	7	37	20	61
missense	5	35	20	52

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	49	24	27
disruptive	21	86	40	101
missense	11	76	28	64

PlasmoDB Total SNPs: 118

Non-coding: 82 | Synonymous: 26 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 441 | Molecular Weight (kDa): 53.327

UniProt ID(s): A5A7N6, Q8I5U1

PDB ID(s): None

Isoelectric Point: 9.55

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007704	GPI mannosyltransferase 1
PFam	PF05007	GPI mannosyltransferase 1
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
17978585	Functional analysis of the first mannosyltransferase (PIG-M) involved inglycosylphosphatidylinositol synthesis in Plasmodium falciparum.	Kim YU, Hong Y	https://pubmed.ncbi.nlm.nih.gov/17978585/
35420475	The Leishmania donovani Ortholog of the Glycosylphosphatidylinositol AnchorBiosynthesis Cofactor PBN1 Is Essential for Host Infection	Roberts A, Nagar R, ..., Wright GJ	10.1128/mbio.00433-22