PF3D7_1211000

Genome location: Pf3D7_12_v3:484,065..489,902(+)

Genome classification: Core

Function and Localization

Product Description: kinesin-X3, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005871	kinesin complex
Function	GO:0005524	ATP binding
Function	GO:0016887	ATP hydrolysis activity
Function	GO:0003777	microtubule motor activity
Process	GO:0007018	microtubule-based movement

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	expressed	0.15	0.92
Schizont	possibly expressed	0.22	0.87
Gametocyte	expressed	0.91	0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1211000

Resistome Missense Mutations: I1531K, I1533K

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.076 | #Insertions: 8

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_060950)

Modification: Tagged | RMgm-5025

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5U0

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.05) with 4UBF (Global RMSD=4.87)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533044

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	25	94	45	81
disruptive	47	150	76	227
missense	28	121	66	198

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	41	160	59	116
disruptive	87	296	168	306
missense	47	235	147	248

PlasmoDB Total SNPs: 181

Non-coding: 36 | Synonymous: 91 | Nonsynonymous: 54 | Stop Codon: 0

Protein Information

Protein Length: 1844 | Molecular Weight (kDa): 214.399

UniProt ID(s): Q8I5U0

PDB ID(s): None

Isoelectric Point: 9.16

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001752	Kinesin motor domain
InterPro	IPR019821	Kinesin motor domain, conserved site
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR027640	Kinesin-like protein
PFam	PF00225	Kinesin motor domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
24737313	Small molecule screen for candidate antimalarials targeting Plasmodium Kinesin-5.	Liu L, Richard J, Kim S, Wojcik EJ	10.1074/jbc.M114.551408