Product Description: DNA helicase MCM8, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0042555 | MCM complex |
| Component | GO:0005656 | nuclear pre-replicative complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0008094 | ATP-dependent activity, acting on DNA |
| Function | GO:0003678 | DNA helicase activity |
| Function | GO:0003688 | DNA replication origin binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Process | GO:0006270 | DNA replication initiation |
| Process | GO:0006271 | DNA strand elongation involved in DNA replication |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.09 | 0.96 |
| Trophozoite | expressed | 0.45 | 0.73 |
| Schizont | expressed | 0.34 | 0.79 |
| Gametocyte | not expressed | 0.49 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1211300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.999 | MFS: -2.907 | #Insertions: 2
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5T7
"Best" AlphaFill ligand hit: 08T ([[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium, Local RMSD=0.06) with 6MII (Global RMSD=1.16)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_104142
Most Similar Human Ortholog: Q9UJA3
TM-align score: 0.54 | RMSD: 6.95
Seq Identity: 0.23 | Length: 632 / 1135
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000125885 | minichromosome maintenance 8 homologous recombination repair factor |
| ENSG00000286235 | novel protein |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 69 | 23 | 74 |
| disruptive | 27 | 95 | 61 | 155 |
| missense | 14 | 83 | 55 | 132 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 30 | 114 | 53 | 82 |
| disruptive | 54 | 223 | 118 | 257 |
| missense | 27 | 185 | 101 | 175 |
PlasmoDB Total SNPs: 247
Non-coding: 163 | Synonymous: 54 | Nonsynonymous: 30 | Stop Codon: 0
Protein Length: 1135 | Molecular Weight (kDa): 132.607
UniProt ID(s): Q8I5T7
PDB ID(s): None
Isoelectric Point: 9.01
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001208 | MCM domain |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR018525 | Mini-chromosome maintenance, conserved site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031327 | Mini-chromosome maintenance protein |
| InterPro | IPR033762 | MCM OB domain |
| InterPro | IPR041562 | MCM, AAA-lid domain |
| PFam | PF00493 | MCM domain |
| PFam | PF17207 | MCM OB domain |
| PFam | PF17855 | MCM, AAA-lid domain |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10872463 | MCM proteins in DNA replication. | Tye BK | 10.1146/annurev.biochem.68.1.649 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |