Product Description: DNA replication licensing factor MCM5, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0042555 | MCM complex |
| Component | GO:0005656 | nuclear pre-replicative complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0008094 | ATP-dependent activity, acting on DNA |
| Function | GO:0003678 | DNA helicase activity |
| Function | GO:0003688 | DNA replication origin binding |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Process | GO:0006270 | DNA replication initiation |
| Process | GO:0006271 | DNA strand elongation involved in DNA replication |
| Process | GO:0000727 | double-strand break repair via break-induced replication |
| Process | GO:0006267 | pre-replicative complex assembly involved in nuclear cell cycle DNA replication |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.44 | 0.73 |
| Schizont | expressed | 0.43 | 0.73 |
| Gametocyte | expressed | 0.26 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1211700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.252 | MFS: -2.757 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0610200)
Modification: Tagged | RMgm-4154
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5T4
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.13) with 4R7Z (Global RMSD=5.09)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102165
Most Similar Human Ortholog: P33992
TM-align score: 0.86 | RMSD: 3.25
Seq Identity: 0.41 | Length: 689 / 758
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100297 | minichromosome maintenance complex component 5 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 31 | 21 | 55 |
| disruptive | 0 | 5 | 5 | 45 |
| missense | 0 | 5 | 4 | 36 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 89 | 21 | 46 |
| disruptive | 1 | 20 | 22 | 84 |
| missense | 0 | 14 | 21 | 48 |
PlasmoDB Total SNPs: 151
Non-coding: 135 | Synonymous: 16 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 758 | Molecular Weight (kDa): 85.859
PDB ID(s): None
Isoelectric Point: 8.2
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001208 | MCM domain |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR018525 | Mini-chromosome maintenance, conserved site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031327 | Mini-chromosome maintenance protein |
| InterPro | IPR033762 | MCM OB domain |
| InterPro | IPR041562 | MCM, AAA-lid domain |
| PFam | PF00493 | MCM domain |
| PFam | PF17207 | MCM OB domain |
| PFam | PF17855 | MCM, AAA-lid domain |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |