Genome location: Pf3D7_12_v3:534,549..536,310(-)
Genome classification: Core
Product Description: glutathione peroxidase-like thioredoxin peroxidase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.11.1.15 (Transferred entry: 1.11.1.24);1.11.1.9 (Glutathione peroxidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004601 | peroxidase activity |
| Function | GO:0008379 | thioredoxin peroxidase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.12 | 0.94 |
| Trophozoite | not expressed | 0.63 | 0.64 |
| Schizont | possibly expressed | 0.20 | 0.87 |
| Gametocyte | not expressed | 0.36 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1212000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.133 | MFS: -3.056 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5T2
"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=4.33) with 2WGR (Global RMSD=2.38)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P36969 | Phospholipid hydroperoxide glutathione peroxidase | OMA | CHEMBL4747331MLS000583955CHEMBL1951048CHEMBL4748785... |
Ortholog Group (OrthoMCL): OG6_100297
Most Similar Human Ortholog: K7ERP4
TM-align score: 0.94 | RMSD: 1.54
Seq Identity: 0.40 | Length: 154 / 205
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000116157 | glutathione peroxidase 7 |
| ENSG00000164294 | glutathione peroxidase 8 (putative) |
| ENSG00000167468 | glutathione peroxidase 4 |
Protein Length: 205 | Molecular Weight (kDa): 23.953
PDB ID(s): None
Isoelectric Point: 9.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000889 | Glutathione peroxidase |
| InterPro | IPR036249 | Thioredoxin-like superfamily |
| PFam | PF00255 | Glutathione peroxidase |
| Superfamily | SSF52833 | Thioredoxin-like superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 7 | 11 | 14 |
| disruptive | 1 | 9 | 8 | 21 |
| missense | 1 | 9 | 8 | 20 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 20 | 11 | 12 |
| disruptive | 2 | 38 | 23 | 47 |
| missense | 1 | 28 | 18 | 36 |
PlasmoDB Total SNPs: 52
Non-coding: 46 | Synonymous: 2 | Nonsynonymous: 4 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11087748 | The putative glutathione peroxidase gene of Plasmodium falciparum codes for athioredoxin peroxidase. | Sztajer H, Gamain B, ..., Flohe L | 10.1074/jbc.M008631200 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21203490 | Compartmentation of redox metabolism in malaria parasites. | Kehr S, Sturm N, ..., Becker K | 10.1371/journal.ppat.1001242 |
| 22889167 | A novel trafficking pathway in Plasmodium falciparum for the organellarlocalization of glutathione peroxidase-like thioredoxin peroxidase. | Chaudhari R, Narayan A, Patankar S | 10.1111/j.1742-4658.2012.08746.x |
| 28462015 | Membrane and luminal proteins reach the apicoplast by different traffickingpathways in the malaria parasite Plasmodium falciparum. | Chaudhari R, Dey V, ..., Patankar S | 10.7717/peerj.3128 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 24637102 | Molecular cloning, characterization and expression profile of a glutathioneperoxidase-like thioredoxin peroxidase (TPxGl) of the rodent malaria parasitePlasmodium berghei | Haselton KJ, David R, ..., Kanzok SM | 10.1016/j.parint.2014.02.004 |