PF3D7_1212100 (PPP1)

Genome location: Pf3D7_12_v3:536,652..538,932(+)

Genome classification: Core

Function and Localization

Product Description: peripheral plastid protein 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0044164	host cell cytosol

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.12	0.94
Trophozoite	possibly expressed	0.74	0.59
Schizont	expressed	0.35	0.80
Gametocyte	possibly expressed	0.63	0.65

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1212100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.143 | MFS: -3.15 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0610600)

Relative Growth Rate: 0.06 ± 0.05
Confidence: 7.39

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5T1

"Best" AlphaFill ligand hit: U5P (uridine-5'-monophosphate, Local RMSD=1.04) with 6DSR (Global RMSD=17.72)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532629

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	36	15	39
disruptive	21	73	32	92
missense	8	59	25	73

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	14	69	29	30
disruptive	46	163	67	154
missense	19	121	49	96

PlasmoDB Total SNPs: 103

Non-coding: 54 | Synonymous: 37 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 558 | Molecular Weight (kDa): 66.487

UniProt ID(s): Q8I5T1

PDB ID(s): None

Isoelectric Point: 10.08

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
18551176	The malaria secretome: from algorithms to essential function in blood stageinfection.	van Ooij C, Tamez P, ..., Haldar K	https://pubmed.ncbi.nlm.nih.gov/18551176/
22144892	A systematic screen to discover and analyze apicoplast proteins identifies aconserved and essential protein import factor.	Sheiner L, Demerly JL, ..., Striepen B	10.1371/journal.ppat.1002392
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895
36332822	Purification, structural characterization and antioxidant activities of twoneutral polysaccharides from persimmon peel	Cui Y, Chen Y, ..., Lu X	10.1016/j.ijbiomac.2022.10.257