PF3D7_1212300

Genome location: Pf3D7_12_v3:543,385..549,255(+)

Genome classification: Core

Function and Localization

Product Description: WD repeat-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005858	axonemal dynein complex
Function	GO:0045504	dynein heavy chain binding
Function	GO:0045503	dynein light chain binding
Process	GO:0003341	cilium movement
Process	GO:0007018	microtubule-based movement

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.18	0.91
Trophozoite	expressed	0.64	0.66
Schizont	expressed	0.12	0.93
Gametocyte	expressed	0.86	0.61

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1212300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -1.224 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5S9

"Best" AlphaFill ligand hit: 1PG (2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol, Local RMSD=1.31) with 5FRE (Global RMSD=37.51)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104291

Most Similar Human Ortholog: H0YDI6

TM-align score: 0.57 | RMSD: 4.47

Seq Identity: 0.22 | Length: 387 / 1251

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000152763	dynein axonemal intermediate chain 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	56	36	88
disruptive	16	108	62	154
missense	16	106	59	138

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	115	64	88
disruptive	44	231	113	236
missense	33	197	101	178

PlasmoDB Total SNPs: 298

Non-coding: 206 | Synonymous: 67 | Nonsynonymous: 25 | Stop Codon: 0

Protein Information

Protein Length: 1251 | Molecular Weight (kDa): 149.219

UniProt ID(s): Q8I5S9

PDB ID(s): None

Isoelectric Point: 9.79

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR036322	WD40-repeat-containing domain superfamily
PFam	PF00400	WD40 repeat
Superfamily	SSF50978	WD40-repeat-containing domain superfamily