About Gene List

PF3D7_1212500 (GAT)

Genome location: Pf3D7_12_v3:551,620..554,600(-)

Genome classification: Core

Function and Localization

Product Description: glycerol-3-phosphate 1-O-acyltransferase

SignalP Peptide: N/A

# Transmembrane Domains: 3

EC Numbers: 2.3.1.15 (Glycerol-3-phosphate 1-O-acyltransferase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005789 endoplasmic reticulum membrane
Component GO:0030176 integral component of endoplasmic reticulum membrane
Function GO:0043136 glycerol-3-phosphatase activity
Function GO:0004366 glycerol-3-phosphate O-acyltransferase activity
Function GO:0016287 glycerone-phosphate O-acyltransferase activity
Process GO:0008654 phospholipid biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.17 0.91
Trophozoite expressed 0.30 0.81
Schizont expressed 0.13 0.91
Gametocyte expressed 0.12 0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1212500

Resistome Missense Mutations: I486T

Resistome Compounds with Missense Mutations: MMV665852

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.161 | MFS: -2.732 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1428500)

  • Relative Growth Rate: 0.10 ± 0.09
  • Confidence: 6.24

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5S7

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.3.1.15 glycerol-3-phosphate 1-O-acyltransferase NEMEDTAMgCl2CaCl2oleatepapainacetonecholatePronaseTrypsinspermineacyl-CoA...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105381

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 3 33 16 52
disruptive 5 16 9 33
missense 5 16 9 31

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 6 113 34 33
disruptive 6 56 40 111
missense 6 49 36 70

PlasmoDB Total SNPs: 124

Non-coding: 100 | Synonymous: 17 | Nonsynonymous: 6 | Stop Codon: 1

Protein Information

Protein Length: 583 | Molecular Weight (kDa): 66.919

UniProt ID(s): Q8I5S7, Q9BH83, Q9BHA5

PDB ID(s): None

Isoelectric Point: 8.69

Protein Domain Annotations:

Source Family ID Description
InterPro IPR002123 Phospholipid/glycerol acyltransferase
PFam PF01553 Phospholipid/glycerol acyltransferase
Superfamily SSF69593 N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
14668349 The Plasmodium falciparum PfGatp is an endoplasmic reticulum membrane proteinimportant for the initial step of malarial glycerolipid synthesis. Santiago TC, Zufferey R, ..., Mamoun CB 10.1074/jbc.M310502200
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104