Product Description: DNA-directed RNA polymerases I, II, and III subunit RPABC3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005736 | RNA polymerase I complex |
| Component | GO:0005665 | RNA polymerase II, core complex |
| Component | GO:0005666 | RNA polymerase III complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003899 | DNA-directed 5'-3' RNA polymerase activity |
| Process | GO:0006352 | DNA-templated transcription, initiation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.32 | 0.84 |
| Trophozoite | expressed | 0.40 | 0.77 |
| Schizont | possibly expressed | 0.08 | 0.95 |
| Gametocyte | not expressed | 0.19 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1213700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.133 | MFS: -3.188 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5R8
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102056
Most Similar Human Ortholog: P52434
TM-align score: 0.83 | RMSD: 2.11
Seq Identity: 0.32 | Length: 132 / 145
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000163882 | RNA polymerase II, I and III subunit H |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 11 | 6 | 5 |
| disruptive | 1 | 2 | 6 | 8 |
| missense | 1 | 2 | 6 | 7 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 17 | 8 | 9 |
| disruptive | 2 | 16 | 7 | 21 |
| missense | 2 | 13 | 5 | 15 |
PlasmoDB Total SNPs: 58
Non-coding: 53 | Synonymous: 5 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 145 | Molecular Weight (kDa): 16.898
UniProt ID(s): Q8I5R8
PDB ID(s): None
Isoelectric Point: 4.78
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005570 | RNA polymerase, Rpb8 |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| PFam | PF03870 | RNA polymerase, Rpb8 |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 11486042 | Partners of Rpb8p, a small subunit shared by yeast RNA polymerases I, II and III | Briand JF, Navarro F, ..., Thuriaux P | 10.1128/MCB.21.17.6056-6065.2001 |
| 11168400 | Intracellular contents and assembly states of all 12 subunits of the RNApolymerase II in the fission yeast Schizosaccharomyces pombe | Kimura M, Sakurai H, Ishihama A | 10.1046/j.1432-1327.2001.01911.x |
| 11168594 | Transcription organization and mRNA levels of the genes for all 12 subunits ofthe fission yeast RNA polymerase II | Sakurai H, Ishihama A | 10.1046/j.1365-2443.2001.00394.x |
| 16730080 | Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei | Nguyen TN, Schimanski B, ..., Gunzl A | 10.1016/j.molbiopara.2006.02.023 |
| 14698245 | Heterologous overexpression and purification of four common subunits of nuclearRNA polymerases I, II and III of Schizosaccharomyces pombe | Proshkin SA, Shpakovski GV | 10.1016/j.jchromb.2003.09.008 |
| 11918671 | Level of the RNA polymerase II in the fission yeast stays constant butphosphorylation of its carboxyl terminal domain varies depending on the phase andrate of cell growth | Sakurai H, Ishihama A | 10.1046/j.1365-2443.2002.00522.x |
| 16621069 | Characterization of RNA polymerase II subunits of Trypanosoma brucei | Devaux S, Lecordier L, ..., Vanhamme L | 10.1016/j.molbiopara.2006.02.020 |