Product Description: proline--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.15 (Proline--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0017101 | aminoacyl-tRNA synthetase multienzyme complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0043906 | Ala-tRNA(Pro) hydrolase activity |
| Function | GO:0004827 | proline-tRNA ligase activity |
| Process | GO:0006433 | prolyl-tRNA aminoacylation |
| Process | GO:0042493 | response to drug |
| Process | GO:0009410 | response to xenobiotic stimulus |
| Process | GO:0006418 | tRNA aminoacylation for protein translation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.44 | 0.78 |
| Trophozoite | expressed | 0.74 | 0.59 |
| Schizont | expressed | 0.12 | 0.92 |
| Gametocyte | expressed | 0.27 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1213800
Resistome Missense Mutations: L482F, T512S
Resistome Compounds with Missense Mutations: Halofuginone, NCP26
Resistome # Samples with Disruptive Mutations: 5 (5 missense, 5 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.106 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1429600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5R7
"Best" AlphaFill ligand hit: BU1 (1,4-butanediol, Local RMSD=0.03) with 7V9D (Global RMSD=0.87)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.15 | proline-tRNA ligase | KClNH4ClPyrroleL-prolineglyburideL-cysteinecysteamine3-pyrrolinePyrrolidinefebrifuginehalofuginoneTCMDC-124506... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100749
Most Similar Human Ortholog: P07814
TM-align score: 0.74 | RMSD: 3.74
Seq Identity: 0.44 | Length: 601 / 746
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000136628 | glutamyl-prolyl-tRNA synthetase 1 |
Protein Length: 746 | Molecular Weight (kDa): 86.647
UniProt ID(s): A0A144A0G8, Q8I5R7
PDB ID(s): 4NCX, 4OLF, 4Q15, 4TWA, 4WI1, 4YDQ, 5IFU, 6T7K, 7F96, 7F97, 7QB7, 7QC1, 7QC2, 7V9D
Isoelectric Point: 7.4
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002314 | Aminoacyl-tRNA synthetase, class II (G/ P/ S/T) |
| InterPro | IPR002316 | Proline-tRNA ligase, class IIa |
| InterPro | IPR004154 | Anticodon-binding |
| InterPro | IPR004499 | Proline-tRNA ligase, class IIa, archaeal-type |
| InterPro | IPR007214 | YbaK/aminoacyl-tRNA synthetase-associated domain |
| InterPro | IPR016061 | Proline-tRNA ligase, class II, C-terminal |
| InterPro | IPR017449 | Prolyl-tRNA synthetase, class II |
| InterPro | IPR033721 | Prolyl-tRNA synthetase, catalytic domain |
| InterPro | IPR036754 | YbaK/aminoacyl-tRNA synthetase-associated domain superfamily |
| PFam | PF00587 | Aminoacyl-tRNA synthetase, class II (G/ P/ S/T) |
| PFam | PF03129 | Anticodon-binding |
| PFam | PF04073 | YbaK/aminoacyl-tRNA synthetase-associated domain |
| PFam | PF09180 | Proline-tRNA ligase, class II, C-terminal |
| Superfamily | SSF52954 | YbaK/aminoacyl-tRNA synthetase-associated domain superfamily |
| Superfamily | SSF55681 | Prolyl-tRNA synthetase, class II |
| Superfamily | SSF55826 | |
| Superfamily | SSF64586 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 36 | 24 | 47 |
| disruptive | 14 | 47 | 29 | 88 |
| missense | 8 | 40 | 23 | 68 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 19 | 75 | 29 | 46 |
| disruptive | 28 | 119 | 82 | 148 |
| missense | 18 | 77 | 62 | 88 |
PlasmoDB Total SNPs: 152
Non-coding: 123 | Synonymous: 19 | Nonsynonymous: 10 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 22355703 | Uneven spread of cis- and trans-editing aminoacyl-tRNA synthetase domains withintranslational compartments of P. falciparum. | Khan S, Sharma A, ..., Sharma A | 10.1038/srep00188 |
| 25047712 | Structural and functional analysis of the anti-malarial drug target prolyl-tRNAsynthetase. | Jain V, Kikuchi H, ..., Yogavel M | 10.1007/s10969-014-9186-x |
| 25395010 | A genomic and evolutionary approach reveals non-genetic drug resistance inmalaria. | Herman JD, Rice DP, ..., Wirth DF | 10.1186/PREACCEPT-1067113631444973 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |