PF3D7_1213900

Genome location: Pf3D7_12_v3:594,197..598,788(-)

Genome classification: Core

Function and Localization

Product Description: W2 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.13 (Mannose-1-phosphate guanylyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005851	eukaryotic translation initiation factor 2B complex
Function	GO:0005085	guanyl-nucleotide exchange factor activity
Function	GO:0003743	translation initiation factor activity
Function	GO:0031369	translation initiation factor binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	2.43	0.17
Trophozoite	possibly expressed	1.28	0.38
Schizont	not expressed	0.57	0.69
Gametocyte	not expressed	1.06	0.46

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1213900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.184 | MFS: -2.806 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1429700)

Relative Growth Rate: 0.14 ± 0.24
Confidence: 4.21

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A0U7

"Best" AlphaFill ligand hit: TDR (thymine, Local RMSD=1.44) with 3FS8 (Global RMSD=28.17)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.13	mannose-1-phosphate guanylyltransferase	GDPGMPEDTAdiphosphateGDP-mannoseGDP-glucosemiltefosineammonium sulfateGDP-6-deoyxmannoseglucose 1-phosphatemannose 1-phosphate2-methyl-9H-pyrido[2,3-b]indole...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101778

Most Similar Human Ortholog: A0A3B3ISM8

TM-align score: 0.79 | RMSD: 2.87

Seq Identity: 0.26 | Length: 357 / 1137

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000145191	eukaryotic translation initiation factor 2B subunit epsilon

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	16	76	45	62
disruptive	36	136	85	185
missense	25	125	78	148

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	26	142	44	56
disruptive	70	287	139	292
missense	52	246	108	202

PlasmoDB Total SNPs: 180

Non-coding: 79 | Synonymous: 65 | Nonsynonymous: 36 | Stop Codon: 0

Protein Information

Protein Length: 1137 | Molecular Weight (kDa): 134.549

UniProt ID(s): A0A144A0U7

PDB ID(s): None

Isoelectric Point: 4.76

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001451	Hexapeptide repeat
InterPro	IPR011004	Trimeric LpxA-like superfamily
InterPro	IPR016024	Armadillo-type fold
InterPro	IPR029044	Nucleotide-diphospho-sugar transferases
PFam	PF00132	Hexapeptide repeat
Superfamily	SSF48371	Armadillo-type fold
Superfamily	SSF51161	Trimeric LpxA-like superfamily
Superfamily	SSF53448	Nucleotide-diphospho-sugar transferases