About Gene List

PF3D7_1214200 (SET5)

Genome location: Pf3D7_12_v3:606,589..608,091(-)

Genome classification: Core

Function and Localization

Product Description: histone-lysine N-methyltransferase SET5

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.43 (Transferred entry: 2.1.1.354)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005739 mitochondrion
Function GO:0018024 histone-lysine N-methyltransferase activity
Process GO:0034968 histone lysine methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.02 0.99
Trophozoite expressed 0.07 0.95
Schizont not expressed 0.00 1.00
Gametocyte not expressed 0.11 0.94

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1214200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.751 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5R3

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.89) with 3KMT (Global RMSD=3.71)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.1.1.43 histone-lysine N-methyltransferase BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_115588

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 3 16 2 15
disruptive 0 25 20 39
missense 0 25 20 36

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 6 20 10 18
disruptive 2 51 25 49
missense 2 49 24 39

PlasmoDB Total SNPs: 37

Non-coding: 27 | Synonymous: 4 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 178 | Molecular Weight (kDa): 20.663

UniProt ID(s): Q8I5R3

PDB ID(s): None

Isoelectric Point: 9.5

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001214 SET domain
InterPro IPR009207 Histone methyltransferase, PBCV type putative
PFam PF00856 SET domain
Superfamily SSF82199 N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
19765590 Potential epigenetic regulatory proteins localise to distinct nuclearsub-compartments in Plasmodium falciparum. Volz J, Carvalho TG, ..., Cowman AF 10.1016/j.ijpara.2009.09.002
33839154 Dynamic association of the H3K64 trimethylation mark with genes encoding exportedproteins in Plasmodium falciparum. Jabeena CA, Govindaraju G, ..., Rajavelu A 10.1016/j.jbc.2021.100614
36975803 Modification of Phosphorylation Sites in the Yeast Lysine Methyltransferase Set5Exerts Influences on the Mitogen-Activated Protein Kinase Hog1 under ProlongedAcetic Acid Stress Ye PL, Yuan B, ..., Zhao XQ 10.1128/spectrum.03011-22