PF3D7_1214300

Genome location: Pf3D7_12_v3:608,122..610,094(-)

Genome classification: Core

Function and Localization

Product Description: geranylgeranyl transferase type-2 subunit beta, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 2.5.1.60 (Protein geranylgeranyltransferase type II)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005968	Rab-protein geranylgeranyltransferase complex
Function	GO:0004663	Rab geranylgeranyltransferase activity
Process	GO:0018344	protein geranylgeranylation
Process	GO:0018342	protein prenylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.10	0.95
Trophozoite	expressed	0.22	0.86
Schizont	expressed	0.04	0.97
Gametocyte	expressed	0.17	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1214300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -3.107 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6S3K4

"Best" AlphaFill ligand hit: FAR (farnesyl, Local RMSD=0.14) with 6J7F (Global RMSD=4.23)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.5.1.60	protein geranylgeranyltransferase type II	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156439

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	27	11	40
disruptive	5	27	26	57
missense	5	25	26	54

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	53	21	36
disruptive	15	94	40	100
missense	8	81	37	64

PlasmoDB Total SNPs: 60

Non-coding: 40 | Synonymous: 18 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 433 | Molecular Weight (kDa): 51.123

UniProt ID(s): C6S3K4

PDB ID(s): None

Isoelectric Point: 9.62

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001330	PFTB repeat
InterPro	IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid
InterPro	IPR026873	Geranylgeranyl transferase type-2 subunit beta
PFam	PF00432	PFTB repeat
Superfamily	SSF48239	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid