Genome location: Pf3D7_12_v3:620,648..622,089(+)
Genome classification: Core
Product Description: thioredoxin peroxidase 2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.11.1.15 (Transferred entry: 1.11.1.24)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0008379 | thioredoxin peroxidase activity |
| Process | GO:0045454 | cell redox homeostasis |
| Process | GO:0006979 | response to oxidative stress |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.15 | 0.90 |
| Schizont | expressed | 0.26 | 0.82 |
| Gametocyte | expressed | 0.56 | 0.69 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1215000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.171 | MFS: -2.937 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1430800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1430800)
Modification: Disrupted | RMgm-3891
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5Q6
"Best" AlphaFill ligand hit: DTU ((2r,3s)-1,4-dimercaptobutane-2,3-diol, Local RMSD=0.02) with 3SBC (Global RMSD=1.03)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.11.1.15 | peroxiredoxin | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q06830 | Peroxiredoxin-1 | OrthoDB | CHEMBL574646 |
Ortholog Group (OrthoMCL): OG6_100111
Most Similar Human Ortholog: Q06830
TM-align score: 0.95 | RMSD: 1.24
Seq Identity: 0.40 | Length: 196 / 216
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000117450 | peroxiredoxin 1 |
| ENSG00000117592 | peroxiredoxin 6 |
| ENSG00000123131 | peroxiredoxin 4 |
| ENSG00000165672 | peroxiredoxin 3 |
| ENSG00000167815 | peroxiredoxin 2 |
Protein Length: 216 | Molecular Weight (kDa): 24.68
PDB ID(s): 2C0D
Isoelectric Point: 8.69
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000866 | Alkyl hydroperoxide reductase subunit C/ Thiol specific antioxidant |
| InterPro | IPR019479 | Peroxiredoxin, C-terminal |
| InterPro | IPR024706 | Peroxiredoxin, AhpC-type |
| InterPro | IPR036249 | Thioredoxin-like superfamily |
| PFam | PF00578 | Alkyl hydroperoxide reductase subunit C/ Thiol specific antioxidant |
| PFam | PF10417 | Peroxiredoxin, C-terminal |
| Superfamily | SSF52833 | Thioredoxin-like superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 6 | 9 | 13 |
| disruptive | 1 | 12 | 12 | 23 |
| missense | 1 | 12 | 11 | 22 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 20 | 8 | 19 |
| disruptive | 3 | 42 | 20 | 62 |
| missense | 3 | 34 | 19 | 42 |
PlasmoDB Total SNPs: 81
Non-coding: 63 | Synonymous: 14 | Nonsynonymous: 4 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16879648 | Structural and biochemical characterization of a mitochondrial peroxiredoxin fromPlasmodium falciparum. | Boucher IW, McMillan PJ, ..., Muller S | 10.1111/j.1365-2958.2006.05303.x |
| 25475729 | Crystal structure and solution characterization of the thioredoxin-2 fromPlasmodium falciparum, a constituent of an essential parasitic protein exportcomplex. | Peng M, Cascio D, Egea PF | 10.1016/j.bbrc.2014.11.096 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |