Product Description: glycerol-3-phosphate dehydrogenase [NAD(+)], putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.1.1.8 (Glycerol-3-phosphate dehydrogenase (NAD(+)));1.1.1.94 (Glycerol-3-phosphate dehydrogenase (NAD(P)(+)))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0009331 | glycerol-3-phosphate dehydrogenase complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0004367 | glycerol-3-phosphate dehydrogenase [NAD+] activity |
| Process | GO:0006116 | NADH oxidation |
| Process | GO:0006072 | glycerol-3-phosphate metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.35 | 0.84 |
| Trophozoite | expressed | 0.39 | 0.77 |
| Schizont | expressed | 0.19 | 0.89 |
| Gametocyte | expressed | 0.19 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1216200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.135 | MFS: -3.106 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1431900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1431900)
Modification: Disrupted | RMgm-4300
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5P5
"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.47) with 6PYP (Global RMSD=2.29)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100385
Most Similar Human Ortholog: C9JFA7
TM-align score: 0.95 | RMSD: 1.23
Seq Identity: 0.41 | Length: 144 / 367
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000152642 | glycerol-3-phosphate dehydrogenase 1 like |
| ENSG00000167588 | glycerol-3-phosphate dehydrogenase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 13 | 10 | 27 |
| disruptive | 7 | 18 | 8 | 22 |
| missense | 7 | 18 | 8 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 28 | 9 | 40 |
| disruptive | 14 | 39 | 20 | 67 |
| missense | 12 | 30 | 17 | 54 |
PlasmoDB Total SNPs: 201
Non-coding: 180 | Synonymous: 10 | Nonsynonymous: 11 | Stop Codon: 0
Protein Length: 367 | Molecular Weight (kDa): 41.234
UniProt ID(s): Q8I5P5
PDB ID(s): 1YJ8
Isoelectric Point: 7.27
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006109 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminal |
| InterPro | IPR006168 | Glycerol-3-phosphate dehydrogenase, NAD-dependent |
| InterPro | IPR008927 | 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily |
| InterPro | IPR011128 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminal |
| InterPro | IPR017751 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, eukaryotic |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF01210 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminal |
| PFam | PF07479 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminal |
| Superfamily | SSF48179 | 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |