Product Description: perforin-like protein 2
SignalP Peptide: MNILLKYHLSYIFFFVHLIVINIKC
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0071944 | cell periphery |
| Component | GO:0097708 | intracellular vesicle |
| Component | GO:0016020 | membrane |
| Component | GO:0031982 | vesicle |
| Function | GO:0022829 | wide pore channel activity |
| Process | GO:0046931 | pore complex assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | not expressed | 0.04 | 0.97 |
| Schizont | not expressed | 0.02 | 0.99 |
| Gametocyte | not expressed | 0.45 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1216700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.741 | #Insertions: 2
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1432400)
Modification: Tagged | RMgm-1504
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5P0
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 52 | 20 | 62 |
| disruptive | 43 | 110 | 59 | 132 |
| missense | 26 | 84 | 54 | 116 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 92 | 41 | 70 |
| disruptive | 70 | 221 | 73 | 219 |
| missense | 41 | 175 | 67 | 174 |
PlasmoDB Total SNPs: 123
Non-coding: 0 | Synonymous: 74 | Nonsynonymous: 49 | Stop Codon: 0
Protein Length: 1073 | Molecular Weight (kDa): 124.896
UniProt ID(s): Q8I5P0
PDB ID(s): None
Isoelectric Point: 7.82
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR020864 | Membrane attack complex component/perforin (MACPF) domain |
| PFam | PF01823 | Membrane attack complex component/perforin (MACPF) domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23461714 | A perforin-like protein mediates disruption of the erythrocyte membrane duringegress of Plasmodium berghei male gametocytes. | Deligianni E, Morgan RN, ..., Siden-Kiamos I | 10.1111/cmi.12131 |
| 24602217 | Perforin-like protein PPLP2 permeabilizes the red blood cell membrane duringegress of Plasmodium falciparum gametocytes. | Wirth CC, Glushakova S, ..., Pradel G | 10.1111/cmi.12288 |
| 31413195 | Role of a patatin-like phospholipase in Plasmodium falciparum gametogenesis andmalaria transmission. | Singh P, Alaganan A, ..., Chitnis CE | 10.1073/pnas.1900266116 |
| 32266171 | Plasmodium Perforin-Like Protein Pores on the Host Cell Membrane Contribute inIts Multistage Growth and Erythrocyte Senescence. | Garg S, Shivappagowdar A, ..., Singh S | 10.3389/fcimb.2020.00121 |
| 36840311 | Antimalarial and Cytotoxic Activity of Native Plants Used in Cabo VerdeTraditional Medicine | Essoh AP, Cassiano GC, ..., Romeiras MM | 10.3390/plants12040963 |