Product Description: DNA topoisomerase 6 subunit A
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.99.1.3 (Transferred entry: 5.6.2.2)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000228 | nuclear chromosome |
| Function | GO:0003677 | DNA binding |
| Function | GO:0003916 | DNA topoisomerase activity |
| Function | GO:0016889 | endodeoxyribonuclease activity, producing 3'-phosphomonoesters |
| Function | GO:0005515 | protein binding |
| Process | GO:0042138 | meiotic DNA double-strand break formation |
| Process | GO:0000706 | meiotic DNA double-strand break processing |
| Process | GO:0007131 | reciprocal meiotic recombination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | nd | nd |
| Trophozoite | expressed | nd | nd |
| Schizont | possibly expressed | nd | nd |
| Gametocyte | expressed | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1217100.2
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.926 | MFS: -2.501 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1432700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1432700)
Modification: Disrupted | RMgm-3902
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5N7
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101269
Most Similar Human Ortholog: Q9Y5K1
TM-align score: 0.86 | RMSD: 2.56
Seq Identity: 0.26 | Length: 311 / 327
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000054796 | SPO11 initiator of meiotic double stranded breaks |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 22 | 9 | 25 |
| disruptive | 11 | 45 | 28 | 49 |
| missense | 11 | 44 | 28 | 47 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 35 | 24 | 26 |
| disruptive | 17 | 89 | 43 | 99 |
| missense | 14 | 82 | 37 | 72 |
PlasmoDB Total SNPs: 161
Non-coding: 143 | Synonymous: 11 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 327 | Molecular Weight (kDa): 38.319
UniProt ID(s): Q8I5N7
PDB ID(s): None
Isoelectric Point: 9.72
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002815 | Spo11/DNA topoisomerase VI subunit A |
| InterPro | IPR013049 | Spo11/DNA topoisomerase VI, subunit A, N-terminal |
| InterPro | IPR034136 | Topoisomerase 6 subunit A/Spo11, TOPRIM domain |
| InterPro | IPR036078 | Spo11/DNA topoisomerase VI subunit A superfamily |
| PFam | PF04406 | Spo11/DNA topoisomerase VI, subunit A, N-terminal |
| Superfamily | SSF56726 | Spo11/DNA topoisomerase VI subunit A superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18007598 | A novel plant gene essential for meiosis is related to the human CtIP and theyeast COM1/SAE2 gene | Uanschou C, Siwiec T, ..., Schlogelhofer P | 10.1038/sj.emboj.7601913 |
| 15477092 | Purification, folding, and characterization of Rec12 (Spo11) meiotic recombinaseof fission yeast | Wu H, Gao J, ..., Wahls WP | 10.1016/j.pep.2004.07.012 |