About Gene List

PF3D7_1217200 (MRD1)

Genome location: Pf3D7_12_v3:678,393..682,293(+)

Genome classification: Core

Function and Localization

Product Description: multiple RNA-binding domain-containing protein 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0030686 90S preribosome
Component GO:0016607 nuclear speck
Component GO:0005634 nucleus
Function GO:0003723 RNA binding
Process GO:0045292 mRNA cis splicing, via spliceosome
Process GO:0000381 regulation of alternative mRNA splicing, via spliceosome
Process GO:0034462 small-subunit processome assembly

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 1.43 0.43
Trophozoite expressed 0.95 0.52
Schizont expressed 0.32 0.82
Gametocyte possibly expressed 0.92 0.53

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1217200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 3 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.129 | MFS: -2.915 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1432800)

  • Relative Growth Rate: 0.06 ± 0.05
  • Confidence: 7.23

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A1F1

"Best" AlphaFill ligand hit: GTA (p1-7-methylguanosine-p3-adenosine-5',5'-triphosphate, Local RMSD=0.02) with 6D0Y (Global RMSD=8.11)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102169

Most Similar Human Ortholog: H0YIL2

TM-align score: 0.52 | RMSD: 3.34

Seq Identity: 0.34 | Length: 74 / 1119

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000122965 RNA binding motif protein 19

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 17 53 24 69
disruptive 56 192 91 229
missense 29 150 81 180

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 27 102 46 74
disruptive 124 391 160 326
missense 55 301 129 218

PlasmoDB Total SNPs: 218

Non-coding: 74 | Synonymous: 93 | Nonsynonymous: 51 | Stop Codon: 0

Protein Information

Protein Length: 1119 | Molecular Weight (kDa): 131.113

UniProt ID(s): A0A144A1F1

PDB ID(s): None

Isoelectric Point: 9.58

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000504 RNA recognition motif domain
InterPro IPR034423 Probable RNA-binding protein 19, RNA recognition motif 5
InterPro IPR035979 RNA-binding domain superfamily
PFam PF00076 RNA recognition motif domain
Superfamily SSF54928 RNA-binding domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104