Product Description: GTP-binding protein EngA
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0005525 | GTP binding |
| Function | GO:0003924 | GTPase activity |
| Function | GO:0043022 | ribosome binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.37 | 0.82 |
| Trophozoite | expressed | 0.91 | 0.53 |
| Schizont | expressed | 0.35 | 0.80 |
| Gametocyte | possibly expressed | 0.61 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1217300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.36 | MFS: -2.591 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1432900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A297
"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.15) with 3A1U (Global RMSD=6.12)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 54 | 18 | 47 |
| disruptive | 30 | 87 | 42 | 126 |
| missense | 23 | 74 | 39 | 111 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 82 | 34 | 52 |
| disruptive | 53 | 171 | 89 | 209 |
| missense | 36 | 141 | 73 | 147 |
PlasmoDB Total SNPs: 260
Non-coding: 61 | Synonymous: 143 | Nonsynonymous: 55 | Stop Codon: 1
Protein Length: 961 | Molecular Weight (kDa): 113.429
UniProt ID(s): A0A144A297
PDB ID(s): None
Isoelectric Point: 5.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR006073 | GTP binding domain |
| InterPro | IPR016484 | GTP-binding protein EngA |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032859 | GTPase Der, C-terminal KH-domain-like |
| PFam | PF01926 | GTP binding domain |
| PFam | PF14714 | GTPase Der, C-terminal KH-domain-like |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 29642957 | Characterization of mitochondrion-targeted GTPases in Plasmodium falciparum. | Gupta K, Gupta A, Haider A, Habib S | 10.1017/S0031182018000501 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 36444972 | Molecular dynamics simulation study on Bacillus subtilis EngA: the presence ofMg(2+) at the active-sites promotes the functionally important conformation | Upendra N, Kavya KM, Krishnaveni S | 10.1080/07391102.2022.2151513 |
| 37637111 | The ribosome assembly GTPase EngA is involved in redox signaling in cyanobacteria | Llop A, Bibak S, ..., Contreras A | 10.3389/fmicb.2023.1242616 |
| 38277729 | Crystal structure of GTPase YsxC from Staphylococcus aureus | Biktimirov A, Islamov D, ..., Usachev K | 10.1016/j.bbrc.2024.149545 |
| 33576519 | Interconnected assembly factors regulate the biogenesis of mitoribosomal large subunit | Tobiasson V, Gahura O, ..., Amunts A | 10.15252/embj.2020106292 |
| 33016853 | Molecular dynamics simulation study on Thermotoga maritima EngA: GTP/GDP boundstate of the second G-domain influences the domain-domain interface interactions | N U, S K | 10.1080/07391102.2020.1826359 |
| 30131421 | The Photosystem II Repair Cycle Requires FtsH Turnover through the EngA GTPase | Kato Y, Hyodo K, Sakamoto W | 10.1104/pp.18.00652 |